瘤果黑种草子组合物对豚鼠气管致痉及RAW264.7细胞分泌炎症因子的影响  

Effects of Compositions of Volatile Oil and Flavonoids of Nigella Glandulifera Seeds on Tracheal Spasm of Guinea Pigs and Inflammatory Factors Secreted by RAW264.7 Cells

作　　者：耿东升[1] 张宏涛 马光霞[1] 曹园 

机构地区：[1]新疆军区药品仪器检验所,新疆乌鲁木齐830063

出　　处：《解放军药学学报》2018年第1期1-5,共5页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金;No.30960512

摘　　要：目的观察维药瘤果黑种草子组合物对豚鼠气管致痉及RAW264.7细胞致炎作用的影响。方法取豚鼠气管螺旋条连接于肌张力换能器进行实验,记录各供试药物对乙酰胆碱和组胺致气管条平滑肌张力改变(痉挛)的影响,计算解痉率;培养RAW264.7细胞,通过MTT法,观察供试药物对RAW264.7细胞存活率的影响;以脂多糖刺激,ELISA法分别检测RAW264.7细胞分泌炎症因子IL-6、IL-8和NO的含量,计算供试药物对RAW264.7细胞分泌炎症因子IL-6、IL-8和NO的抑制率。进行较大范围的实验剂量-效应反应筛选,分别确定气管条和RAW264.7细胞实验的各种组合物剂量。实验设正常对照组、阳性对照组(氨茶碱)、单用挥发油和黄酮对照组及组合物(A^I)组。结果对乙酰胆碱致痉作用,组合物C组的解痉率高于阳性对照组(P<0.05),其组合物中黄酮与挥发油剂量之比为1∶10,组合物B、F、G组与阳性对照组无明显差异(P>0.05),组合物B、C、E、F、G、I组均高于单用黄酮和挥发油组(P<0.05);对组胺致痉作用,组合物A、B、C、E组的解痉率与阳性对照组无明显差异(P>0.05),组合物A、C、D、E组均高于单用黄酮和挥发油组(P<0.05)。对于抑制RAW264.7细胞分泌炎症因子IL-6、IL-8的作用,组合物Ⅲ-Ⅸ的抑制率均较单用黄酮和挥发油组高(P<0.05),组合物间比较,组合物Ⅸ抑制率最高,其组合物中黄酮与挥发油剂量之比为80∶25。结论瘤果黑种草子组合物的抑痉和抑泌作用优于单用瘤果黑种草子挥发油或瘤果黑种草子黄酮,实验效应与组合物中挥发油和黄酮的剂量比呈现一定的对应关系。Objective To observe the effects of compositions of the volatile oil and flavonoids extracted from Nigella Glandulifera seeds（NVFC）in Uygur＇s herbs on tracheal spasm of guinea pigs and on inflammatory factors secreted by RAW264.7 cells.Methods As the first step,the tracheal spiral strips from guinea pigs were obtained before they were connected to the transducer of muscular tension.The effect of trial drugs on the tension changes（or spasm）of the tracheal smooth muscles induced by acetylcholine（ACh）and histamine（His）was tested and recorded.The spasmolytic rates of the trial drugs to the tracheal smooth muscles induced by ACh and His were calculated.Secondly,RAW264.7 cells were cultured,and the effects of the trial drugs on the survival rates of RAW264.7 cells were observed by the method MTT.With LPS stimulation,the levels of IL-6,IL-8 and NO excreted by RAW264.7 cells in trial drugs were examined using ELISA.The inhibitory rates to the inflammatory factors in the trial drugs were calculated.Finally,the experimental drug doses of the tracheal strips and RAW264.7 cells were respectively defined by experimental screening of the reactions of drug doses to the trial effects.Results In terms of spasmogenic action of ACh,the spasmolytic rates of the NVFC group C were better than those of positive control.The dose ratio of flavonoids to the volatile oil was 1∶10,and there was no significant difference between the BFG of NVFC group and that of positive control（P〈0.05）.B,C,E,F,G,I of NVFC group was stronger than that of groups of single volatile oil and flavonoids（P〈0.05）.As far as spasmogenic action of His was concerned,the spasmolytic rates of A,B,C,E of NVFC group were similar to those positive control（P〈0.05）,but A,C,D,E of NVFC group was stronger than that of groups of single volatile oil and flavonoids（P〈0.05）.In respect of inhibitory rates of IL-6 and IL-8 secreted by RAW264.7 cells,Ⅲ andⅨof NVFC group were better than those of groups of single volatile oil and flavonoi

关 键 词：瘤果黑种草子 气管痉挛 炎症因子 

分 类 号：R965[医药卫生—药理学]