显齿蛇葡萄片中二氢杨梅素含量测定方法  

Determination of Dihydromyricetin in Ampelopis Grossedentata Tablets

作　　者：张木子荷 张诗龙[1] 范娜[1] 宋杨[1] 徐平华[1] 韩晋[1] 

机构地区：[1]解放军302医院药学部,北京100039

出　　处：《解放军药学学报》2018年第1期64-65,70,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：军队科研专项"制剂标准提高";No.13ZJZ12

摘　　要：目的建立显齿蛇葡萄片中二氢杨梅素含量的HPLC测定方法。方法采用HPLC法对显齿蛇葡萄片中二氢杨梅素进行含量测定。选用Zorbax-SB C18色谱柱(4.6mm×250mm,5μm);以甲醇-0.05%磷酸水溶液(35∶65)为流动相;检测波长:289nm;流速:1.0ml·min^(-1);柱温:30℃。结果二氢杨梅素在10.2~102.1μg·ml^(-1)的范围内有良好的线性关系(r=0.9998),平均回收率97.6%,RSD为0.87%。结论该方法简便、准确,重复性好,精密度高,可用于显齿蛇葡萄片中二氢杨梅素的含量测定。Objective To establish an HPLC method for the determination of dihydromyricetin in ampelopis grossedentata tablets.Methods High performance liquid chromatography was employed to determine the content of dihydromyricetin.The mobile phase was a mixture of methonal and 0.05% phosphoric acid（35∶65）.The flow rate was 1.0·min^-1 and the detection wavelength was 289 nm.Results The linear relationship was good within the concentration range of 10.2-102.1μg·^-1（r =0.9998）.The average recovery was 97.6%（RSD=0.87%）.Conclusion This method is accurate and simple with good specificity and reproducibility.It can be used in the content determination of ampelopis grossedentata tablets.

关 键 词：显齿蛇葡萄片 二氢杨梅素 HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]