EAMG大鼠胸腺、下丘脑-垂体-肾上腺皮质轴的病理变化及补脾强力复方的干预作用研究  

Pathological changes of the thymus and the hypothalamic-pituitary-adrenocorticalaxis in rats with experimental autoimmune myasthenia gravis and the interventional effect of compound spleen-tonifying prescription

作　　者：曾陈西 王静[1] 况时祥[2] 楚兰 张艺凡 何前松[2] 赵芝兰[2] 况耀鋆 ZENG Chen- xi1 , WANG Jing1 , KUANG Shi - xiang2, CHU Lan3 , ZHANG Yi - fan3, HE Qian - song2, ZHAO Zhi - lan2, KUANG Yao - jun2(1. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou, China; 2. The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China; 3. The Affiliated Hospital of Guizhou Medical University,Guiyang 550025 ,Guizhou,Chin)

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵阳中医学院第二附属医院,贵州贵阳550003 [3]贵州医科大学附属医院,贵州贵阳550025

出　　处：《湖南中医杂志》2018年第2期130-133,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81360605)

摘　　要：目的:探讨实验性自身免疫性重症肌无力(Experimental Autoimmune Myasthenia Gravis,EAMG)模型大鼠胸腺下丘脑-垂体-肾上腺皮质(HPA)轴的病理变化情况及补脾强力复方对其的干预作用。方法:SPF级雌性Lewis大鼠72只,随机分为正常组、模型组、强的松组及补脾强力复方大、中、小剂量组,每组各12只。对除正常组外的其余组大鼠制备EAMG模型。通过HE染色法观察EAMG大鼠胸腺、HPA轴的病理变化;药物干预后观察各组大鼠胸腺、HPA轴功能的差异。结果:与正常组相比,EAMG大鼠出现了胸腺细胞滤泡性增生,形成较多生发中心以及淋巴滤泡;肾上腺皮质变薄,束状带细胞萎缩,形成囊状空泡及腺瘤样改变;下丘脑神经细胞有不同程度的细胞变性,出现胶质细胞增生、细胞核固缩或消失,形成空泡;垂体神经细胞嗜酸性细胞呈弥漫性或结节性增生,偶见腺瘤样变。与各相应模型组对比,补脾强力复方组EAMG大鼠的胸腺、HPA轴的病理变化范围及程度减轻。结论:EAMG大鼠的胸腺、肾上腺、下丘脑、垂体可见病理性变化,而具有补脾益肾、温扶阳气作用的补脾强力复方对上述病理变化有一定的干预作用,其中在保护EAMG大鼠肾上腺形态功能上的作用较优。Objective ： To investigate the pathological changes of the thymus and the hypothalamic - pituitary - adreno- cortical （HPA） axis in rats with experimental autoimmune myasthenia gravis （EAMG） and the interventional effect of compound spleen -tonifying prescription. Methods：A total of 72 specific pathogen- free female Lewis rats were randomly divided into normal group, model group, prednisone group, and high - , medium - , and low - dose compound spleen - toni- lying prescription groups,with 12 rats in each group. All rats except those in the normal group were used to establish a model of EAMG. HE staining was used to observe the pathological changes of the thymus and the HPA axis ; the functions of the thymus and the HPA axis were compared between groups after drug intervention. Results：Compared with the normal rats, the EAMG rats had follicular hyperplasia of thymocytes, the formation of more germinal centers and lymphoid follicles ; the adrenal cortexbecame thinner, and showed atrophy of fascicular cells, with the formation of cystic vacuoles and adenomatoid changes;the hypothalamic neurons showedvarying degrees of cell degeneration, with glial cell proliferation, karyopyknosis or disappearance of nuclei, and the formation of vacuoles ; pituitary eosinophils showed diffuse or nodular hyperplasi- a,with occasional adenomatoid changes. Compared with the model group, the compound spleen -tonifying prescription groups had significant reductions in the extent and degree of the pathological changes of the thymus and the HPA axis. Conclusion：EAMG rats have visible pathological changes of the thymus, the adrenal gland, the hypothalamus, and the pituitary gland. Compound spleen - tonifying prescription has spleen - tonifying, kidney - nourishing, and yang - supporting effects and thus exerts a certain interventional effect on these pathological changes, especially a better effect in protecting the morphology and function of the adrenal gland in EAMG rats.

关 键 词：补脾强力复方 HPA轴 胸腺 实验研究 

分 类 号：R285.5[医药卫生—中药学]