斑马鱼胚胎发育过程中内源性多肽的表达及与眼发育的相关研究  

Expression of endogenous peptides during zebrafish embryonic development and its correlation with eye development

作　　者：李楠[1,2] 张昕[1] 史春梅[1] 张青宇[1,2] 郭锡熔 池霞[1,2] 童梅玲[1] 

机构地区：[1]南京医科大学附属妇产医院儿童保健科,江苏南京210004 [2]南京医科大学儿科研究所,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2018年第2期161-165,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81670900;81600779);江苏省"六大人才高峰"第七批高层次人才项目(2010001);南京市重点科学和科技发展计划(ZKX16061);南京医科大学科技发展项目(2015NJMU092)

摘　　要：目的:研究斑马鱼发育过程中内源性多肽的表达规律以及与眼发育的相关性。方法:分别收集0、48 h斑马鱼胚胎进行多肽质谱分析,筛选发育过程中的差异多肽,利用生物信息学方法分析差异多肽对应的前体蛋白及前体基因,从中筛选与眼发育相关的内源性多肽。结果:研究共筛选得到差异多肽232条。理化特性分析发现分子量均分布在2 000 Da以下,等电点主要以弱酸和弱碱性为主,中性极少。生物信息学发现Vitellogenin(VTG)、Titin(TTN)基因编码的前体蛋白富集较多与胚胎发育和眼发育相关的差异多肽。信号通路分析(ingenuity pathway analysis,IPA)发现差异多肽的分子功能和生理系统功能与胚胎发育相关,差异多肽富集信号通路包括轴突介导的信号通路、FAK信号通路和MAPK信号通路,这些信号通路与眼发育密切相关。结论:斑马鱼胚胎期发育过程中内源性差异多肽理化特性稳定,与胚胎发育密切相关,并可能通过相关信号通路来影响眼发育,将为眼发育提供新的研究思路,为机制研究奠基基础。Objective：To identify the expression of endogenous peptides during development of zebrafish and their relationship with eye development. Methods：The zebrafish embryos were collected for 0 hour and 48 hours respectively for peptide mass spectrometry analysis. The differential peptides in the development process were screened for bioinformatics analysis to detect the relationship with eye development. Results：A total of 232 bands of differential peptides were screened. Analysis of physical and chemical properties found that the molecular weight is distributed below 2 000 Da,isoelectric point of proteins mainly weak acid and weak alkaline,isoelectric point of little protein is neutral. Bioinformatics analysis found that Vitellogenin（VTG）,Titin（TTN）encoded precursor protein have large numbers of peptides. The molecular function and physiological function of all differential peptides were analyzed by ingenuity pathway analysis（IPA）,which found they are closely related embryonic development. The signal pathway analysis revealed that axonal-mediated signaling pathway,FAK signal pathway and MAPK signal pathway related to eye development. Conclusion：The differential endogenous peptides of zebrafish embryo development are stable,closely related to embryonic development,and may affect eye development through related signal pathway,which will provide new research ideas and mechanism for eye development.

关 键 词：内源性多肽 眼发育 斑马鱼 液相色谱-串联质谱分析 信号通路 

分 类 号：Q813.1[生物学—生物工程]