Mn^(2+)、Pb^(2+)对野生垂穗披碱草种子萌发与幼苗生长的影响  

Effects of Mn^(2+) and Pb^(2+) on seed germination and seedling growth of Elymus nutans

作　　者：徐雅梅[1] 王传旗[1] 武俊喜[2] 张文静 王小川 赤列催珍 徐德飞 包赛很那 苗彦军[1] 

机构地区：[1]西藏农牧学院,西藏林芝860000 [2]中国科学院地理科学与资源研究所,北京100101

出　　处：《草业学报》2018年第3期194-200,共7页Acta Prataculturae Sinica

基　　金：国家重点研发计划项目(2016YFC0502002);西藏自治区科技厅重点项目(2016ZDKJZC-15);国家自然基金(31560142)资助

摘　　要：研究了西藏巴青县野生垂穗披碱草种子萌发与幼苗生长对重金属Mn^(2+)、Pb^(2+)胁迫的生理响应机制,为西藏矿区周边污染土壤和植被修复提供依据。选取西藏巴青县野生垂穗披碱草为试验材料,采用纸上发芽法研究了重金属Mn^(2+)、Pb^(2+)对其种子萌发和幼苗生长的影响。结果表明,Mn^(2+)、Pb^(2+)离子胁迫对垂穗披碱草种子萌发和幼苗生长具有极显著的影响(P<0.01);随着Mn^(2+)、Pb^(2+)浓度的增大,垂穗披碱草种子发芽率、发芽势、发芽指数、活力指数及胚芽长表现为下降的趋势;在200 mg·L^(-1) Mn^(2+)、Pb^(2+)溶液浓度培养下垂穗披碱草种子根较对照略长;随着Mn^(2+)、Pb^(2+)溶液浓度的升高,垂穗披碱草幼苗叶片叶绿素a和叶绿素b含量持续降低而电导率和MDA含量呈上升趋势。垂穗披碱草对Mn^(2+)、Pb^(2+)具有一定的耐受性,可适用于Mn^(2+)、Pb^(2+)轻度污染土壤。In order to provide reference for contaminated soil and vegetation restoration in the Tibetan mining area, a study has been undertaken of the physiological response of Elymus nutans ＇ seed germination and seedling growth to heavy metal Mn2＋ and Pb2＋ stress in Baqing county, Tibet. Select wild E. nutans from the county were used as test materials for investigation using germination methods. The results showed that Mn2＋ and Pb2＋ ion stress had a significant effect on seed germination and seedling growth. With increasing Mn2＋ and Pb2＋ concentrations, the germination percentage, germination vigor, germination index, vigor index and bud length of E. nutans showed a declining trend. Under a solution concentration of 200 mg . L-1 Mn2＋ and Pb2＋ , the shoot length of E. nutans was slightly longer than that of the control. With increasing Mn2＋ and Pb2＋ concentrations, the contents of chlorophyll a and chlorophyll b in leaves decreased while electrical conductivity and malondialdehyde content showed a rising trend. This study suggests that E. nutans has some tolerance to Mn2＋ and Pb2＋ ion stress that makes it a useful application for soils that are mildly contaminated with these toxins.

关 键 词：垂穗披碱草 种子萌发 幼苗生长 Mn2+、Pb2+ 巴青县 

分 类 号：S543.9[农业科学—作物学]