外源NO对玉米幼苗抗旱生理指标的影响  

Effects of Exogenous NO on the Drought Resistance Physiological Indexes of Maize Seeding Leaves under Drought Stress

作　　者：徐新娟[1,2] 王永志 李兆举[1] 单长卷 

机构地区：[1]河南科技学院,河南新乡453003 [2]现代生物育种河南省协同创新中心,河南新乡453003 [3]三门峡职业技术学院,河南三门峡472000

出　　处：《灌溉排水学报》2018年第3期41-44,共4页Journal of Irrigation and Drainage

基　　金：河南省教育厅科学技术研究重点项目(13A180302);河南科技学院国家级大学生创新创业训练项目(201610467003)

摘　　要：【目的】明确外源NO在玉米抗旱中的应用效果。【方法】采用PEG-6000模拟干旱胁迫,硝普钠(SNP)为NO供体,研究了外源NO对干旱胁迫条件下玉米幼苗叶片主要抗旱生理指标的影响。【结果】干旱胁迫显著提高了叶片过氧化氢(H2O2)、丙二醛(MDA)、谷胱甘肽(GSH)及Vc量(p<0.05),增强了谷胱甘肽过氧化物酶(GPX)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性,同时显著降低了超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性(p<0.05)。不同浓度SNP对GSH量无显著影响,但显著降低H2O2和MDA的量(p<0.05);50μmol/L和100μmol/L SNP分别使Vc量显著增加了13.4%和16.1%(p<0.05),但150μmol/L SNP对Vc量无显著影响;不同浓度SNP均显著提高了干旱胁迫下SOD、CAT、GPX和APX活性(p<0.05)。【结论】因此,外源NO增强了玉米幼苗的抗氧化能力,减轻了干旱所造成的氧化胁迫,提高了玉米幼苗的抗旱性。[Objective] Identify the application effect of exogenous NO on drought resistance of maize.[Method] The effects of exogenous NO on the main drought resistance physiological indexes of maize seedlings leaf under drought stress were studied by using PEG-6000 simulated drought stress and sodium nitrate （SNP） as NO donor. [Result] Drought stress significantly increased the content of leaf hydrogen peroxide （H202）, malondialdehyde （MDA）, glutathione （GSH） and ascorbic acid （Vc）（p〈0.05）, and enhanced the activity of glutathione peroxidase （GPX）, ascorbate peroxidase （APX） and glutathione reductase （GR）; the drought stress significantly decreased the activity of superoxide dismutase （SOD） and catalase （CAT） （p〈0.05）. Different SNP concentrations had no significant effect on glutathione （GSH） content, but significantly reduced the content of H202 and MDA（p〈0.05）. The Vc content of 50 μmol/L and 100 μmol/L SNP was significantly increased by 13.4% and 16.1% （p〈0.05）, respectively, but 150 μmol/L SNP had no significant effect on Vc content. Moreover, the activity of SOD, CAT, GPX and APX were significantly increased under different concentrations of SNP （/）〈0.05）. [Conclusion] Therefore, exogenous NO can enhance the drought resistance of maize seedlings by improving the antioxidant ability and alleviating the oxidative stress caused by drought.

关 键 词：一氧化氮 干旱胁迫 氧化胁迫 抗氧化酶 玉米 

分 类 号：S513.01[农业科学—作物学]