有偿献血HCV高危人群IL-6水平与HCV RNA及肝功能的相关性研究  

Study on correlation of the plasma IL-6 concentration with HCV RNA and liver function in HCV high-risk paid blood donors

作　　者：杨静静 黄鹏[2] 田亭[2] 凡豪志 喻荣彬[2] 岳明[3] 张云[2,4] 王洁 

机构地区：[1]河海大学医院预防保健科,江苏南京210098 [2]南京医科大学公共卫生学院流行病与卫生统计学系,江苏南京211166 [3]南京医科大学第一附属医院感染病科,江苏南京210029 [4]南京军区军事医学研究所流行病学室,江苏南京210002 [5]南京医科大学护理学院基础与社区护理学系,江苏南京211166

出　　处：《中华疾病控制杂志》2018年第2期165-167,172,共4页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81473028;81473029;81502853);江苏省自然科学基金(BK20151026);江苏省重点学科建设项目"护理学"(JX10617801);江苏高校"青蓝工程"

摘　　要：目的了解有偿献血丙型肝炎病毒(hepatitis C virus,HCV)高危人群血浆白细胞介素6(interleukin6,IL-6)水平与HCV RNA病毒载量及肝功能指标的相关性,探讨细胞因子在HCV感染慢性化机制中的作用。方法采用酶联免疫吸附法(enzyme-linked immunosorbent,ELISA)检测HCV持续感染者(138例)和对照组未感染者(152例)的血浆IL-6水平,采用荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)法检测其HCV RNA水平,采用Spearman相关和偏相关分析以上指标间的相关性。结果 HCV慢性感染者的血浆IL-6水平高于对照组(t=-3.066,P=0.002);IL-6与丙氨酸氨基转移酶(alanine aminotransferase,ALT)和天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)水平均呈正相关(调整前:r_(ALT)=0.186,P_(ALT)=0.001,r_(AST)=0.177,P_(AST)=0.003;调整后:r_(ALT)=0.200,P_(ALT)=0.001,r_(AST)=0.127,P_(AST)=0.039);IL-6与HCV RNA水平呈正相关(调整前r=0.199,P=0.019;调整后r=0.200,P=0.025)。结论血浆IL-6可能与HCV慢性感染密切相关,宿主免疫在HCV感染的慢性化机制中可能发挥着不可忽视的作用。Objective To explore the relationship of plasma interleukin 6 （IL-6） concentration with HCV RNA, liver function in hepatitis C patients and investigate the role of cytokines in chronic HCV infection. Methods 138 patients with persistent HCV infection and 152 uninfected controls were selected in this study. The plasma IL-6 level and HCV viral load were detected by enzyme linked immunosorbent assay （ELISA） and fluorescence quantitative polymerase chain reaction （FQ-PCR） respectively. The spearman correlation and partial correlation analyses were used to explore their correlations with liver function indexes. Results The chronic HCV infected patients had higher plasma IL-6 level than that of healthy controls （ t = - 3. 066, P = 0. 002）. The IL-6 concentration had a positive correlation with alanine aminotransferase （ALT）, aspartate aminotransferase （AST） （before the adjustment： r ALT =0. 186, PALT = 0. 001, rAST = 0. 177. PAST = 0. 003 ; after the adjustment ： rALT = 0. 200, PALT = 0. 001, FAST = 0. 127, PAST = 0. 039）. The HCV viral load was positive- ly correlated with the IL-6 level in chronic hepatitis C patients （before the adjustment： r = 0. 199, P = 0. 019; after the adjustment ： r = 0. 200, P = 0. 025 ）. Conclusions The plasma IL-6 may be associated with HCV infection and chronic level. The findings provided more insights into the immune mechanism for the development of HCV infection.

关 键 词：丙型肝炎病毒 慢性病 白细胞介素6 病毒载量 丙氨酸氨基转移酶 

分 类 号：R373.21[医药卫生—病原生物学] R392.114[医药卫生—基础医学]