PRMT5对卵巢癌细胞增殖及侵袭转移能力的影响  

Effect of PRMT5 on proliferation,migration and invasion of ovarian cancer cells

作　　者：包香香 张瑞 王子超 李红[1] 陈芳[1] 

机构地区：[1]潍坊市人民医院妇科,潍坊265100

出　　处：《现代妇产科进展》2018年第2期90-94,共5页Progress in Obstetrics and Gynecology

基　　金：山东省医药卫生科技发展计划项目(No:2016WS0653)

摘　　要：目的:研究精氨酸甲基转移酶5(PRMT5)对卵巢癌A2780及SKOV3细胞增殖及侵袭转移能力的影响,探讨其可能机制。方法:设计并体外合成靶向PRMT5的siRNA序列(si P)及阴性对照序列(si C),分别转染卵巢癌细胞A2780、SKOV3。Brd U掺入实验检测细胞增殖能力;Transwell小室技术检测细胞迁移和侵袭能力;Western blot法检测PRMT5降调后E-cadherin蛋白表达变化。结果:转染si P后,卵巢癌细胞中PRMT5表达明显降低。A2780细胞中,si P组的Brd U阳性率明显低于si C组[(11.7±1.5)%vs(33.3±1.5)%,P<0.001];SKOV3细胞中,si P组的Brd U阳性率明显低于si C组[(18.0±2.0)%vs(35.3±1.5)%,P<0.001]。A2780和SKOV3细胞中,si P组的迁移和侵袭细胞数均显著少于si C组和N组,差异均有统计学意义(P<0.001)。Western blot法显示,转染后72h,si P组中E-cadherin蛋白表达明显上调。结论:PRMT5参与了卵巢癌细胞的生长增殖和迁移侵袭的过程,干扰其表达能显著减慢细胞的增殖,减弱其迁移和侵袭能力。PRMT5可能通过调控E-cadherin表达参与卵巢癌细胞的迁移和侵袭过程。Objective： To investigate the effect of protein arginine methyltransferase 5（ PRMT5）-siRNA-mediated gene silencing on proliferation,migration and invasion of ovarian cancer cell line A2780 and SKOV3. Methods： The human PRMT5-siRNA（ si P） and controlsiRNA（ si C） sequences were chemically synthesized and were transfected into ovarian cancer cell line A2780 and SKOV3 respectively.PRMR5 was down-regulated successfully. Brd U incorporation was used to detect the proliferation activity and transwell assay was employed to test migration and invasion of ovarian cancer cells after si P was transfection.E-cadherin protein was detected by Western blot. Results： PRMT5 protein was down-regulated significantly after si P transfected in ovarian cancer cells. Compared to control sample（ si C）,cells treated with si P showed significantly decreased Brd U incorporation,in both A2780 and SKOV3（ P 0. 001,respectively）.In migration and invasion transwell assays,the number of cells with si P group was lower than that of si C and N groups（ P0.001,respectively）.E-cadherin protein was up-regulated after si P transfection in ovarian cancer cells after 72 h. Conclusion： PRMT5-siRNA could significantly inhibit the proliferation,migration and invasion in ovarian cancer cell A2780 and SKOV3,suggest that PRMT5 could play an important role in the tumorigenesis and development of ovarian cancer.Maybe,PRMT5 was involved in migration and invasion of ovarian canser cells via the expression of E-cadherin.

关 键 词：卵巢癌 精氨酸甲基转移酶5 小干扰RNA 生长增殖 迁移 侵袭 

分 类 号：R711[医药卫生—妇产科学]