枸杞多糖对心肌梗死大鼠心肌细胞凋亡的影响  

Effect of Lycium Barbarum Polysaccharides on theapoptosis of cardiocytes in myocardial infarction rat

作　　者：李丽芳[1] 杜娟[1] 薛华[1] 李晓霞[1] 

机构地区：[1]宁夏银川市第一人民医院,宁夏银川750001

出　　处：《宁夏医学杂志》2018年第2期97-99,共3页Ningxia Medical Journal

基　　金：宁夏高等学校科技研究项目资金(NGY2014108)

摘　　要：目的探讨枸杞多糖对心肌细胞凋亡的抑制作用及其机制。方法选取正常SD大鼠60只(雄性,体重200~250 g),随机分为空白对照组(n=15)、心肌梗死组(n=15)、枸杞多糖组(n=15)、氯沙坦组(n=15)。心肌梗死组、枸杞多糖组、氯沙坦组大鼠通过结扎左冠状动脉前降支,建立大鼠心肌梗死模型;枸杞多糖组、氯沙坦组分别给予枸杞多糖120 mg/(kg·d)及氯沙坦(20 mg/(kg·d))每日1次灌胃干预治疗4周;空白对照组、心肌梗死组给予等容量生理盐水灌胃。4周后用25%乌拉坦以4 m L/kg腹腔注射麻醉后处死大鼠,制备心肌病理标本及心肌匀浆,用TUNNEL法检测心肌凋亡细胞、Western blotting方法检测心肌Fas蛋白水平。结果心肌梗死组细胞凋亡指数、Fas水平较空白对照组明显增加(P<0.05),枸杞多糖组、氯沙坦组细胞凋亡指数、Fas水平较心肌梗死组明显下降(P<0.05),两药物组之间比较差异无统计学意义(P>0.05)。结论枸杞多糖能够抑制心肌梗死大鼠心肌细胞的凋亡,其作用可能与降低心肌Fas蛋白表达有关。Objective To observe the effect of Lycium Barbarum Polysaccharides （LBP） on theapoptosis of cardiocytes in my- ocardial infarction rat in order to discuss the possible mechanism. Methods 60 SD rats were randomly divided into the control group, the model group（the myocardial infarction group）, LBP group and Losartan group （n = 15）. LBP group were given LBP 120 mg/kg by gavage, Losartan group was given Losartan 20 mg./kg by gavage, the control group and model group were given the same amount of sa- line, which were given for 4 weeks, once per day. The apoptosis was assessed by TUNEL assay. The expression of Fas was detected by Western Blot and Immunohistochemistry methods. Results Theapoptosis rate of cardiocytes and Fas protein expression in the model group were significantly higher than the control group（P 〈0.05）. Theapoptosis rate of cardiocytes and Fas protein expression in the LBP group were significantly lower than the model group（ P 〈 0.05 ）. There was no significantly different between the LBP group and Losartan group. Conchmion LBP can reduce theapoptosis of cardiocytes and inhibit the expression of Fas protein. It has a protective role in myo- cardial infarction.

关 键 词：枸杞多糖 心肌梗死 细胞凋亡 FAS蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]