高效液相色谱法测定藿砂口服液中茅术醇和β-桉叶醇的含量  

Determination of Hinesol and β-eudesmol in Huosha Oral Liquid by HPLC

作　　者：李劭 翁淑琴[1] 林雄[1] 潘旭东[1] 严国鸿[1] 

机构地区：[1]福建中医药大学附属人民医院,福州30004

出　　处：《海峡药学》2018年第2期43-45,共3页Strait Pharmaceutical Journal

基　　金：福建省科技厅引导性项目(2015Y0060)

摘　　要：目的研究并建立高效液相色谱法测定藿砂口服液中茅术醇和β-桉叶醇的含量。方法以乙腈-0.1%磷酸溶液梯度洗脱为流动相;所使用的色谱柱为Diamonsi L C18(2)(5μm,250×4.6mm);柱温:30℃,进样量:10μL,流速:1.0m L·min-1,检测波长为203nm。结果按以上实验方法,茅术醇和β-桉叶醇分别在0.0182mg·m L-1~0.4548mg·m L-1(r=0.9998)和0.0153mg·m L-1~0.3834mg·m L-1(r=0.9997)浓度范围内与其峰面积具有良好的线性关系。茅术醇和β-桉叶醇的平均回收率分别为96.76%和96.89%,RSD分别为1.13%和1.83%(n=6)。结论所述方法结果准确,可以作为检测藿砂口服液中茅术醇和β-桉叶醇含量的方法并用于该制剂的质量控制。OBJECTIVE To research and establish an HPLC method to determine the content of hinesol and β-eudesmol in Huosha oral liquid. METHODS Acetonitrile-0. 1% phosphoric acid solution as the mobile phase gradient elution;The column used was Diamonsil C18（2）（5μm,250 × 4. 6 mm）;Column temperature：30℃,injection volume：10μL,Velocity of flow：1. 0 m L·min^-1,Detection wavelength of 203 nm. RESULTS By the above experimental methods,a good linear correlation was observed within the range of 0. 0182 mg · m L^-1~ 0. 4548 mg · m L^-1 for Hinesol（ r = 0. 9998） and 0. 0153 mg·m L^-1~ 0. 3834 mg·m L^-1 for β-eudesmol（ r = 0. 9997）. Hinesol and β-eudesmol average recoveries（ n = 6） were 96. 76% and 96. 89%（ RSD were 1. 13% and 1. 83%）. CONCLUSION The method above is accurate. It can be used as content determination method of hinesol and β-eudesmol in Huosha oral liquid and used to control the quality of Huosha oral liquid.

关 键 词：藿砂口服液 茅术醇 Β-桉叶醇 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]