Large variability in ambient ozone sensitivity across 19 ethylenediurea-treated Chinese cultivars of soybean is driven by total ascorbate  

作　　者：Lijun Jiang Zhaozhong Feng Lulu Dai Bo Shang Elena Paoletti 

机构地区：[1]State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China [2]State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China [3]College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China [4]National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy

出　　处：《Journal of Environmental Sciences》2018年第2期10-22,共13页环境科学学报（英文版）

基　　金：supported by State Key Laboratory of Soil and Sustainable Agriculture(No.Y20160030);the Hundred Talents Program,Chinese Academy of Sciences,Chinese Academy of Sciences President's International Fellowship Initiative(PIFI)for Senior Scientists(Grant Number 2016VBA057);CNR-CAS bilateral agreement 2017–2019(Ozone impacts on plant ecosystems in China and Italy)

摘　　要：The sensitivity of Chinese soybean cultivars to ambient ozone（O3） in the field is unknown,although soybean is a major staple food in China. Using ethylenediurea（EDU） as an O3 protectant, we tested the gas exchange, pigments, antioxidants and biomass of 19 cultivars exposed to 28 ppm·hr AOT40（accumulated O3 over an hourly concentration threshold of40 ppb） over the growing season at a field site in China. By comparing the average biomass with and without EDU, we estimated the cultivar-specific sensitivity to O3 and ranked the cultivars from very tolerant（〈 10% change） to highly sensitive（〉 45% change）, which helps in choosing the best-suited cultivars for local cultivation. Higher lipid peroxidation and activity of the ascorbate peroxidase enzyme were major responses to O3 damage, which eventually translated into lower biomass production. The constitutional level of total ascorbate in the leaves was the most important parameter explaining O3 sensitivity among these cultivars. Surprisingly, the role of stomatal conductance was insignificant. These results will guide future breeding efforts towards more O3-tolerant cultivars in China, while strategies for implementing control measures of regional O3 pollution are being implemented. Overall, these results suggest that present ambient O3 pollution is a serious concern for soybean in China, which highlights the urgent need for policy-making actions to protect this critical staple food.The sensitivity of Chinese soybean cultivars to ambient ozone（O3） in the field is unknown,although soybean is a major staple food in China. Using ethylenediurea（EDU） as an O3 protectant, we tested the gas exchange, pigments, antioxidants and biomass of 19 cultivars exposed to 28 ppm·hr AOT40（accumulated O3 over an hourly concentration threshold of40 ppb） over the growing season at a field site in China. By comparing the average biomass with and without EDU, we estimated the cultivar-specific sensitivity to O3 and ranked the cultivars from very tolerant（〈 10% change） to highly sensitive（〉 45% change）, which helps in choosing the best-suited cultivars for local cultivation. Higher lipid peroxidation and activity of the ascorbate peroxidase enzyme were major responses to O3 damage, which eventually translated into lower biomass production. The constitutional level of total ascorbate in the leaves was the most important parameter explaining O3 sensitivity among these cultivars. Surprisingly, the role of stomatal conductance was insignificant. These results will guide future breeding efforts towards more O3-tolerant cultivars in China, while strategies for implementing control measures of regional O3 pollution are being implemented. Overall, these results suggest that present ambient O3 pollution is a serious concern for soybean in China, which highlights the urgent need for policy-making actions to protect this critical staple food.

关 键 词：Ground-level ozone Glycine max CULTIVAR Ozone sensitivity ANTIOXIDANTS Gas exchange Biomass Total ascorbate 

分 类 号：S565.1[农业科学—作物学] X515[环境科学与工程—环境工程]