Electrochemical and spectroelectrochemical characterization of different mesoporous TiO2 film electrodes for the immobilization of Cytochrome c  

作　　者：Stavros KATSIAOUNIS Christina TIFLIDIS Christina TSEKOURA Emmanuel TOPOGLIDIS 

机构地区：[1]Department of Materials Science, University of Patras, Rion 26504, Greece

出　　处：《Frontiers of Materials Science》2018年第1期64-73,共10页材料学前沿（英文版）

摘　　要：In this work three different mesoporous TiO2 film electrodes were prepared and used for the immobilization of Cytochrome c （Cyt-c）. Films prepared via a standard sol-gel route （SG-films） were compared with commercially available benchmark nanotitania materials, namely P25 Degussa （P25-films） and Dyesol nanopaste （Dyesol films）. Their properties, film deposition characteristics and their abilities to adsorb protein molecules in a stable and functional way were examined. We investigated whether it is possible, rather than preparing TiO2 films using multistep, lengthy and not always reproducible sol-gel procedures, to use commercially available nanotitania materials and produce reproducible films faster that exhibit all the properties that make TiO2 films ideal for protein immobilization. Although these materials are formulated primarily for dye-sensitized solar cell applications, in this study we found out that protein immobilization is facile and remarkably stable on all of them. We also investigated their electrochemical properties by using cyclic voltammetry and spectroelectrochemistry and found out that not only direct reduction of Fe（III）-heme to Fe（II）-heme of immobilized Cyt-c was possible on all flms but that the adsorbed protein remained electroactive.In this work three different mesoporous TiO2 film electrodes were prepared and used for the immobilization of Cytochrome c （Cyt-c）. Films prepared via a standard sol-gel route （SG-films） were compared with commercially available benchmark nanotitania materials, namely P25 Degussa （P25-films） and Dyesol nanopaste （Dyesol films）. Their properties, film deposition characteristics and their abilities to adsorb protein molecules in a stable and functional way were examined. We investigated whether it is possible, rather than preparing TiO2 films using multistep, lengthy and not always reproducible sol-gel procedures, to use commercially available nanotitania materials and produce reproducible films faster that exhibit all the properties that make TiO2 films ideal for protein immobilization. Although these materials are formulated primarily for dye-sensitized solar cell applications, in this study we found out that protein immobilization is facile and remarkably stable on all of them. We also investigated their electrochemical properties by using cyclic voltammetry and spectroelectrochemistry and found out that not only direct reduction of Fe（III）-heme to Fe（II）-heme of immobilized Cyt-c was possible on all flms but that the adsorbed protein remained electroactive.

关 键 词：TiO2 films SPECTROELECTROCHEMISTRY cyclic voltammetry Cytochrome c 

分 类 号：O643.36[理学—物理化学] Q559.9[理学—化学]