百脉根LjSERKs基因家族在根瘤共生中的功能鉴定  

Identifying the function of LjSERKs gene family in root nodule symbiosis

作　　者：汪涛 冯勇 张忠明[1] WANG Tao, FENG Yong, ZHANG Zhongming(State Key Laboratory Agricultural Microbiology/College of Life Science ＆ Technology, Huazhong Agricultural University ,Wuhan 430070, Chin)

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2018年第2期9-15,共7页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31670240)

摘　　要：为探索百脉根中的体细胞胚胎发生受体类蛋白激酶基因(somatic embryogenesis receptor-like kinases,SERKs)家族在根瘤共生过程中的功能和作用机制,根据拟南芥AtSERKs基因序列,从百脉根基因组中鉴定到8个同源基因(LjSERKs)。利用CRISPR-Cas9基因组编辑和毛根转化技术,分别敲除LjSERKs家族不同基因,观察并分析其对共生结瘤表型的影响。结果显示:敲除LjSERK2、LjSERK3、LjSERK6和LjSERK7后,其植株结瘤数与对照植株相比均无明显差异;敲除LjSERK8后,植株结瘤数显著减少。这些结果表明LjSERK8参与根瘤共生过程,而该家族其他基因间可能存在功能冗余现象。Somatic embryogenesis receptor protein kinase gene（SERKs）belongs to the family of genes encoding LRR receptor kinase and participates in multiple life activities including plant growth,development and immunity.Eight homologous genes（LjSERKs）were identified from Arabidopsis thaliana AtSERKs gene sequence to explore the function and mechanism of SERKs gene family in the process of nodule symbiosis.Using the CRISPR-Cas9 genome editing and hair root transformation technique,the different genes of the LjSERKs family were knocked out separately.The effects on the symbiotic nodule phenotype were observed and analyzed.The results showed that the number of nodules was not different significantly from that of the control plants after knockouting LjSERK2,LjSERK3,LjSERK6 and LjSERK7.The number of nodules was significantly decreased after knockouting LjSERK8.It is indicated that LjSERK8 is involved in the nodule symbiosis process and there may be functional redundancy between other genes in the family.

关 键 词：百脉根 SERKs基因家族 CRISPR-Cas9技术 共生结瘤 

分 类 号：S182[农业科学—农业基础科学] Q78[生物学—分子生物学]