人源化抗Her2单抗对BT474人乳腺癌细胞的抗癌效应研究  

Anti-cancer Effect of Humanized Anti-Her2 Monoclonal Antibody on BT474 Human Breast Cancer Cells

作　　者：杨军[1,2] 王珊珊 杨德宣[2] 李琳娜[2] 马增春[1,2] 袁守军 

机构地区：[1]安徽医科大学研究生院,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《解放军药学学报》2017年第5期400-403,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技重大专项新药临床前药效学评价技术平台项目;No.2012ZX09301003-001

摘　　要：目的评价人源化抗Her2单抗(MIL41)注射液体内外对人乳腺癌细胞BT474的抗癌效应。方法流式细胞仪分析人乳腺癌BT474细胞的Her2表达量;采用CCK-8法测定MIL41体外对BT474细胞的增殖抑制效应;用裸鼠皮下移植瘤模型法,评价MIL41的体内抗癌作用。结果流式细胞仪显示BT474细胞为Her2高表达的细胞系;体外实验显示,MIL41对受试细胞的增殖具有良好的抑制作用;体内实验表明,MIL41对裸鼠皮下移植瘤生长具有较强的抑制作用,呈现良好的量效关系,在给药剂量为12mg·kg-1时,瘤重抑制率达到56.3%(P<0.05)。结论人源化抗Her2单抗体内外对人乳腺癌细胞BT474具有良好的抑制作用。Objective To evaluate the anti-cancer effect of humanized anti-Her2 monoclonal antibody（MIL41）injection on human breast cancer cell line BT474 in vitroandin vivo.Methods Her2 expression in human breast cancer cell BT474 was analyzed by flow cytometry.The inhibitory effect of MIL41 on proliferation of BT474 cells was evaluated using CCK-8 method.The subcutaneous transplantation tumor model of nude mice was adopted to evaluate the anti-cancer effect of MIL41 in vivo.Results Flow cytometry showed that BT474 cells were highly-expressed Her2 cell lines.MIL41 was effective in inhibiting the proliferation of the cells in vitro.The result in vivo experiments showed that the growth of transplanted tumor in nude mice was well inhibited in a dose-ependent manner.When the dosage was 12 mg·kg-1,the inhibition rate of tumor weight was 56.3%（P〈0.05）.Conclusion Humanized anti-Her2 monoclonal antibody has a significant inhibitory effect on human breast cancer cell BT474 in vitro andin vivo.

关 键 词：人源化单抗 MIL41 Her2 抗肿瘤 

分 类 号：R965[医药卫生—药理学]