湿润生肌膏对肛瘘术后创面肉芽组织中碱性成纤维细胞生长因子mRNA表达的影响  

Influence of Shengji ointment on expression of basic fibroblast growth factor mRNA in granulation tissue after operation of anal fistula

作　　者：王世霞[1] 陈永乐[1] 王亚儒 吕高波[1] 

机构地区：[1]陕西省宝鸡市中心医院肛肠外科,721008

出　　处：《检验医学与临床》2018年第5期658-660,664,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨湿润生肌膏对肛瘘手术患者术后创面肉芽组织中碱性成纤维细胞生长因子mRNA表达的影响。方法选取2016年1月至2016年10月于该院接受手术治疗的低位单纯性肛瘘患者95例作为研究对象,随机分为观察组、对照组与凡士林组;在术后不同时期分别提取3组患者的创面组织总RNA,通过Poly A Tract(r)mRNA Isolation System系统进行分离纯化后,使用反转录-聚合酶链反应(RT-PCR)观察碱性成纤维细胞生长因子mRNA的表达变化,并比较不同组别患者的疗效。结果观察组术后7、14、21d的创面愈合率分别为(51.72±4.33)%、(63.85±6.26)%、(95.51±2.79)%,与对照组及凡士林组比较差异有统计学意义(P<0.05);观察组痊愈29例(90.63%),显效3例(9.37%),治疗效果明显优于对照组及凡士林组患者(P<0.05);术后7d时,3组患者碱性成纤维细胞生长因子mRNA阳性值最高,而观察组患者镜下血管内皮细胞、成纤维细胞与单核细胞等大量细胞的细胞质中出现碱性成纤维细胞生长因子mRNA阳性表达区域多于其他两组。结论在肛瘘术后应用湿润生肌膏治疗对创面肉芽组织中碱性成纤维细胞生长因子mRNA的表达有影响,在创口愈合的过程中,碱性成纤维细胞生长因子mRNA的阳性表达可以促进修复细胞的迁移与增殖,加速创面的修复能力。Objective To explore the efficacy of Shengji ointment on basic fibroblast growth factor mRNA expression of granulation tissue of patients with anal fistula after surgery.Methods From January 2016 to October 2016,95 patients with simple low anal fistula in our hospital accepted surgical treatment were selected as the research subjects.And they were randomly divided into observation group,control group and vaseline group in different period.After the total RNA was extracted from the tissue of the three groups of patients,RNA was purified by Poly A Tract(R)from mRNA isolation system.Meanwhile,using RT-PCR method,the expression of basic fibroblast growth factor mRNA was observed,and the effect of different groups of patients were compared.Results Wound healing rates of seven days,14 days and 21 days after surgery of observation group were(51.72±4.33)%,(63.85±6.26)% and(95.51±2.79)%,respectively.Compared with the control group and the vaseline group,differences were statistically significant(P<0.05).A total of 29 cases were cured(90.63%)and 3 cases were effective(9.37%)in the observation group,and the treatment effect was better than the control group and the vaseline group patients(P<0.05).Seven days after surgery,basic fibroblast growth factor mRNA expressions of three groups patients were the strongest,and the basic fibroblast growth factor mRNA expression of cytoplasm of vascular endothelial cells,fibroblasts and mononuclear cells of the observation group were higher than the other two groups.Conclusion The application of moist Shengji ointment treatment has effect on the expression of basic fibroblast growth factor mRNA in wound granulation tissue in anal fistula after operation.In the process of wound healing,the positive expression of basic fibroblast growth factor mRNA could promote the migration and proliferation of repair cells and accelerate wound repair ability.

关 键 词：湿润生肌膏 肛瘘 成纤维细胞 生长因子 mRNA 

分 类 号：R266[医药卫生—中医外科学]