深圳地区金葡菌杀白细胞毒素基因检测及分子流行病学研究  

Prevalence of panton-valentine leukocidin gene in staphylococcus aureus and its molecular epidemiology in shenzhen

作　　者：周小海 龚文波 

机构地区：[1]广东省深圳市人民医院检验科,518020

出　　处：《白求恩医学杂志》2018年第1期8-9,共2页Journal of Bethune Medical Science

基　　金：深圳市2015年科技研发资金基础研究项目(编号:JCYJ20150403101146287)

摘　　要：目的研究深圳市多所医院临床分离金葡菌中杀白细胞毒素(PVL)分布特征及与临床疾病的关系。方法对深圳市14所医院临床分离的724株金葡菌进行PCR检测PVL基因,纸片扩散法检测耐甲氧西林金黄色葡萄球菌(MRSA)表型,mec A^fem B双重PCR确证MRSA基因型,并对PVL阳性的MRSA进行葡萄球菌染色体盒子(SCCmec)分型。结果 724株金葡菌中49株(49/724,6.8%)携带PVL基因;PVL阳性门诊菌株7.1%(4/56),住院6.7%(45/668),差异无统计学意义(P>0.05);PVL阳性的MRSA经SCCmec分型,Ⅱ型6.7%(3/45),III型4.2%(9/215),未分型1.9%(2/105),差异无统计学意义(P>0.05)。结论携带PVL的金葡菌可引起严重侵蚀性感染,及时检测并做同源性分析,利于控制医院感染暴发。Objective To investigate the distribution of panton-valentine leukocidin( PVL) gene in S. aureus,and its relation with clinical diseases. Methods PVL gene was detected by PCR in 724 strains of S. aureus isolated from 14 hospitals in Shenzhen. Methicillin-resistant S. aureus( MRSA) carrying PVL gene phenotype was determined by K-B method,and were confirmed by mec A ~ fem B double PCR. SCCmec typing was confirmed by multiplex PCR. Results Forty-nine among 724 strains of S. aureus carried PVL gene( 6. 8%). The average prevalence of PVL was 7. 1% and 6. 7% in outpatients and inpatients respectively. The difference had no statistic significance( P > 0. 05). SCCmec typing was conducted for PVL-positive MRSA isolates. The prevalence of SCCmec II,SCCmec III and nontypable strains accounted for 6. 7%,4. 2%,and 1. 9% respectively( P > 0. 05). Conclusion S. aureus carrying PVL can result in serous erosion infection,timely inspection and analysis are effective in controlling infection outbreak in hospital.

关 键 词：杀白细胞毒索 金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 

分 类 号：R378.11[医药卫生—病原生物学]