转甜菜碱醛脱氢酶基因增强羽衣甘蓝的耐盐性研究  

Salinity Tolerance in Transgenic Kale Expressing the Betaine Aldehyde Dehydrogenase Gene

作　　者：李春枝 潘彩霞 刘宏伟 刘冰 罗晓明 李大红 

机构地区：[1]驻马店市园林绿化科研所,河南驻马店463000 [2]驻马店市绿化处,河南驻马店463000 [3]黄淮学院生物与食品工程学院,河南驻马店463000

出　　处：《北方园艺》2018年第5期33-38,共6页Northern Horticulture

基　　金：河南省高校重点资助项目(16A210032)

摘　　要：羽衣甘蓝(Brassica oleracea L.var.accephala DC.)是目前常用观赏植物。该研究中,菠菜BADH(甜菜碱醛脱氢酶)基因用农杆菌介导转入了羽衣甘蓝中,共获得了7株转基因植株,并经PCR、Southern blot和实时荧光定量PCR分析。结果表明:BADH基因已整合到羽衣甘蓝基因组并在转基因植物中表达。在盐胁迫下,转基因植物中的BADH酶活性明显高于野生型植物,而转基因植株的MDA含量及相对电导率明显低于野生型植物。表明BADH活性增加,膜的渗透性保护能力也增强,转基因植物的抗逆性增加。同时,在盐胁迫下转基因羽衣甘蓝植株平均主根长、鲜质量、成活率和叶绿素含量均显著高于野生植株,而干质量/鲜质量明显低于野生植株。转基因羽衣甘蓝耐盐性明显增强,该转基因植物可以为羽衣甘蓝抗性育种提供分子基础。Kale（Brassica oleracea L.var.accephala DC.）is one of the most popular and widely ornamental plants in the world.In this study,BADH（betaine aldehyde dehydrogenase）gene derived from spinach was transformed into the genome of kale mediated by Agrobacterium tumefaciens.Seven transgenic plants were obtained,and PCR,Southern blot and RT-qPCR analyses were conducted.The results showed that the BADH gene was integrated into kale genome,transcribed and expressed in the transgenic plants.The BADH enzymes activity of transgenic plants were tested after treating with NaCl stress.It showed that the average values of the activity of BADH enzymes in transgenic plants were higher than that of WT plants,and there was a significant difference between transgenic and WT.Furthermore,MDA content and the average value of relative electronic conductivity of transgenic plants were significantly lower than that of WT plants,which indicated that the protection ability of membrane penetration was enhanced when BADH activity increasing and the stress resistance of the transgenic plants was improved by introduction BADH gene into kale.At the same time,the transgenic kale plants were vigorously growing under salt stress.The average main root length,fresh weight,survival rate and chlorophyll content of transgenic plants were significantly higher than that of WT plants,while dry/fresh weight of transgenic plants was obviously lower than that of WT plants.Those results showed that the traits of salt tolerance of transgenic kale plants were improved after transforming with BADH gene,and these transgenic plants would be used as fundamental germplasm for stress-tolerant breeding of kale.

关 键 词：羽衣甘蓝 BADH基因 转基因植物 耐盐性 

分 类 号：S635.903.6[农业科学—蔬菜学]