濒危藏药材喜马拉雅紫茉莉组织培养初步研究  

Preliminary study on the tissue culture of Mirabilis himalaica(Edgew.) Heim.

作　　者：刘兰[1] 刘继[2] 张勇仓[1] 次仁久米 苏嘉琪 洛桑索朗 罗布卓玛 赤列白觉 

机构地区：[1]西藏大学医学院,拉萨850000 [2]成都市农林科学院 [3]四川农业大学,成都611130

出　　处：《微量元素与健康研究》2018年第2期41-44,共4页Studies of Trace Elements and Health

基　　金：西藏自治区高校青年教师创新支持计划项目(QCZ2016-04);西藏大学大学生创新训练项目(2015QCX056);西藏大学珠峰学者人才发展支持计划项目;2015年西藏大学医学院青年科学培育基金项目

摘　　要：以濒危藏药材喜马拉雅紫茉莉为实验对象,初步建立了喜马拉雅紫茉莉的再生体系,为其种质资源保护和育种工作奠定了一定的理论基础。以常规方式诱导种子萌发,发现其萌发率低,而脱去外种皮后其种子萌发率可达100%。以无菌苗子叶节为外植体进行愈伤组织诱导,最佳激素组合为0.5 mg/L 6-BA+1.5mg/LNAA。以愈伤组织进行不定芽诱导,最佳激素组合为0.5 mg/L 6-BA+1.5 mg/L IAA及0.5 mg/L 6-BA+0.5 mg/L IBA。Objective To provide theoretical basis for the genetic resource conservation and breeding of Mirabilis himalaica（Edgew.）Heim.,its regeneration system was established Preliminaryly.When Mirabilis himalaica（Edgew.） Heim.＇s seeds were induced to germinate by conventional methods,their germination rates were low,but the rates could be 100% when their testae were sloughed.Callus were induced from the cotyledonary nodes of aseptic seedling,the optimum hormone treatment was 0.5 mg/L 6-BA ＋ 1.5 mg/L NAA.Adventitious buds were induced from callus,the optimum hormone treatment was 0.5 mg/L 6-BA ＋ 1.5 mg/L IAA and 0.5 mg/L 6-BA ＋ 0.5 mg/L IBA.

关 键 词：喜马拉雅紫茉莉 组织培养 不定芽 子叶节 

分 类 号：R284.2[医药卫生—中药学]