17β-雌二醇对卵巢切除小鼠海马内脑源性神经营养因子及神经营养因子 3表达的影响(英文)  

Effect of 17β-estradiol on the expression of brain-derived neurotrophic factor and neurotrophin-3 in the hippocampus of ovariectomized mice

作　　者：聂伟[1] 张永祥[1] 周文霞[1] 

机构地区：[1]北京药理毒理研究所,北京100850

出　　处：《中国药理学与毒理学杂志》2002年第4期246-249,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家重点基础研究发展规划项目资助课题(G19990 5 44 0 1);军队杰出人才基金项目资助课题~~

摘　　要：目的　为确定雌激素可能具有的神经营养调节作用。方法　采用蛋白质印迹法检测上述指标的变化。结果　与卵巢未切除对照组相比 ,小鼠卵巢切除 10周后海马内脑源性神经营养因子 (BDNF)表达水平明显下降 ,给予 17β 雌二醇 (2 .4或 4 .8μg·d- 1,sc ,连续 12周 )替代具有明显的改善作用 (P <0 .0 1)。但卵巢切除及 17β 雌二醇替代对海马内神经营养因子 3表达水平无明显影响。结论　海马内BDNF表达水平的改变与雌激素缺乏具有密切关系。雌激素对调节海马内BDNF水平具有重要作用。AIM To confirm the possible neurotrophomodulatory effect of estrogens. METHODS Western blot analysis was employed to determine the levels of brain-derived neurotrophic factor(BDNF) and neurotrophin 3(NT-3). RESULTS There was a significant reduction of BDNF expression in the hippocampus of ovariectomized mice compared with that of sham-operated mice (P<0.01). Replacement of 17β-estradiol(2.4 or 4.8 μg·d -1, sc, for 12 weeks) restored BDNF level in the hippocampus. However, both ovariectomy and estradiol replacement had no effect on NT-3 expression in the hippocampus. CONCLUSION The decrease in the expression of BDNF in the hippocampus of ovariectomized mice is closely associated with estrogen deficiency. Estrogen plays an important role in modulating BDNF level in the hippocampus.

关 键 词：卵巢切除术 17Β-雌二醇 小鼠 海马 内脑源性神经营养因子 神经营养因子3 

分 类 号：R965[医药卫生—药理学] R977.12[医药卫生—药学]