高致病性禽腺病毒4型中国流行株在LMH细胞中的增殖规律  

Replication pattern of highly pathogenic FAdV-4 Chinese epidemic strain in LMH cells

作　　者：刘延珂 万文妍 王贝贝 吴艳阳 杨东东[1] 高冬生[1] 李永涛[1] 王新卫[1] 常洪涛[1] 陈陆[1] 王川庆[1] 赵军[1] YANG Xia 

机构地区：[1]河南农业大学牧医工程学院

出　　处：《畜牧与兽医》2018年第3期82-86,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划(2016YFD0501102,2016YFD0500802);河南省高校科技创新团队支持计划(14IRTSTHN015)

摘　　要：为研究禽腺病毒4型（FAd V-4）中国流行株在鸡肝癌上皮细胞系（LMH）中的生长特性和增殖规律,分别利用SYBR Green I实时荧光定量PCR和病毒滴度测定方法检测FAd V-4感染LMH细胞后12、24、36、48、60、72、84、96和120 h的基因组复制和病毒粒子增殖动态。结果显示,FAd V-4中国流行株在LMH中能够很好增殖,不同时间点的病毒滴度与病毒基因组拷贝数呈正相关,病毒收获的最佳时间为感染后60 h。研究结果为防控鸡肝炎-心包积液综合征疫苗的研发和生产提供一定的依据和参考。In order to understand the growth characteristics and replication pattern of fowl adenovirus serotype 4（ FAd V-4） Chinese epidemic strain in the chicken hepatocellular carcinoma cell line（ LMH）,the dynamics of FAd V-4 genome and viral particle replications in LMH at 12 h,24 h,36 h,48 h,60 h,72 h,84 h,96 h and 120 h after infection was investigated using the SYBR Green I real-time quantitative PCR and virus titration. The results showed that FADV-4 replicated very well in LMH cells,and the virus titers at different time points were positively correlated with the numbers of viral genome copies. The optimal time point for harvesting virus was at 60 h after infection. The present study provides some basic guidelines for development of vaccines to prevent and control hepatitis and hydropericardium syndrome.

关 键 词：禽腺病毒4型 鸡肝癌上皮细胞系 SYBR Green I实时荧光定量PCR 增殖规律 

分 类 号：S855.3[农业科学—临床兽医学]