银杏叶提取物对小鼠脑缺血再灌注损伤的拮抗作用  

Protective effects of extract from leaves of Ginkgo biloba on focal cerebral ischemia-reperfusion injury in mice

作　　者：廖红[1] 高静[1] 张祖暄[1] 徐强[2] 

机构地区：[1]南京大学医学院,江苏南京210093 [2]南京大学医药生物技术国家重点实验室,江苏南京210093

出　　处：《中国药理学与毒理学杂志》2002年第4期273-276,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：江苏省自然科学基金资助项目 (BK930 30 11)~~

摘　　要：目的　研究银杏叶提取物 (EGb76 1)对小鼠脑缺血再灌注损伤的拮抗作用及其作用机理。方法双侧颈总动脉加迷走神经结扎法 (左侧不完全结扎 )造成小鼠局部脑缺血 5min ,再灌注 30min。DTNB法测定谷胱甘肽过氧化物酶 (GSH px)活性 ;荧光分光光度法测定脑组织线粒体膜蛋白结合铽 ;被动性回避行为学模型检测小鼠记忆能力 ;海马形态学观察。结果　小鼠局部脑缺血再灌注后 ,海马GSH px活性降低 ,海马 ,下丘脑和皮质线粒体膜蛋白结合铽降低 ,说明结合钙升高 ,海马CA1区神经元损伤 ,小鼠的记忆能力下降。EGb76 1(2 5 ,5 0 ,10 0mg·kg- 1·d- 1,ig ,连续 7d)能逆转上述变化。结论　EGb76AIM To investigate whether the extract from leaves of Ginkgo biloba(EGb761) can protect neurons from focal cerebral ischemia-reperfusion injury in mice. METHODS Focal cerebral ischemia(5 min)-reperfusion(30 min) injury was induced by occlusion of common carotid artery and vagus nerve(the left was occluded incompletely). The activity of glutathione peroxidase(GSH-px) of hippocampus was determined by the method of DTNB. The mitochondrial membrane protein-bound Tb 3+ fluorescent intensity was measured by fluorospectrophotometry. One-trial passive avoidance model was carried out for determining memory retention in mice. Morphological observation of hippocampus was made with a light microscopy. RESULTS After cerebral ischemia-reperfusion, GSH-px activity in hippocampus was decreased and mitochondrial membrane protein-bound Tb 3+ fluorescent intensity in hippocampus, hypothalamus and cortex decreased, which indicated the increase in protein-bound Ca 2+. Hippocampus CA1 neurons were damaged and memory retention was alleviated. However, the pretreatment with EGb761(25, 50, 100 mg·kg -1·d -1, ig for 7 d) reduced the above changes. CONCLUSION EGb761 Protects neurons from focal cerebral ischemia-reperfusion injury in mice by virtue of anti-oxidation and inhibition of Ca 2+ overloading.

关 键 词：谷胱甘肽过氧化物酶 线粒体膜蛋白结合铽 银杏叶提取物 小鼠 脑缺血 再灌注损伤 

分 类 号：R285.5[医药卫生—中药学] R286.32[医药卫生—中医学]