通心络对高脂血症兔血管内皮功能的保护作用及机制研究  

Protective Effect and its Mechanism of Tongxinluo on Vascular Endothelial Function in Hyperlipidemic Rabbits

作　　者：尹玉洁 张倩[1,2,3] 贾振华 张军芳[2] 王宏涛 魏聪[2] 常丽萍 孙永辉 李彩云 

机构地区：[1]河北中医学院研究生学院,石家庄050090 [2]河北以岭医药研究院,石家庄050035 [3]河北医科大学附属以岭医院心血管病科,石家庄050091

出　　处：《中国中西医结合杂志》2018年第3期325-330,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)(No.2012CB518606);河北省杰出青年科学基金(No.H2015106063)

摘　　要：目的探讨通心络对高脂血症兔血管内皮功能的影响。方法将48只新西兰兔随机分为对照组、模型组、通心络低、中、高剂量组和阿托伐他汀组,每组8只。对照组给予普通饲料,模型组及药物干预组给予高脂饮食建立高脂血症模型。造模同时分别给予各药物干预组通心络胶囊0.15、0.3、0.6 g/kg,阿托伐他汀2.5mg/kg灌胃,每日1次,连续4周。生化法检测各组血脂(TC、TG、HDL-C、LDL-C)水平变化,ELISA法和硝酸还原酶法检测血清内皮素-1(ET-1)和一氧化氮(NO)水平,HE染色观察动脉形态学变化,离体血管环检测主动脉内皮舒张功能,采用蛋白免疫印记(Western blot)检测动脉PI3K、AKT、p-AKT蛋白表达。结果与对照组比较,模型组血清TC、TG、HDL-C、LDL-C和ET-1水平升高(P<0.01),NO水平及主动脉舒张率下降(P<0.01),颈动脉组织PI3K、p-AKT蛋白表达量下调(P<0.01);与模型组比较,通心络高剂量组和阿托伐他汀组血清TC、TG、LDL-C水平降低,通心络中剂量组血清TG、LDL-C水平降低(P<0.05,P<0.01);通心络高、中剂量组和阿托伐他汀组血清ET-1水平下降,NO水平及主动脉舒张率升高(P<0.05,P<0.01),颈动脉组织PI3K、p-AKT蛋白表达量上调(P<0.05,P<0.01)。结论通心络能够改善高脂血症兔的血管内皮依赖性舒张功能,具体机制与其激活PI3 K/AKT信号转导通路相关。Objective To observe the influences of Tongxinluo on vascular endothelial function and its related mechanisms. Methods Totally 48 New Zealand rabbits were randomly divided into the control group, the model group, the low-dose Tongxiniuo group, the medium-dose Tongxinluo group, the high-dose Tongxinluo group and the Atorvastatin group, 8 in each group. Rabbits in the control group were normally fed, while those in model group and drug intervention groups were fed with high-fat diet in to establish hyperlipidaemia model, While molding, rabbits in each drug intervention group were respectively offered Tongxinluo Capsules 0.15, 0, 3 and 0.6 g/kg and Atorvastatin 2.5 mg/kg by gastrogavage, once a day, for consecutive four weeks. Blood lipid （TC, TG, HDL-C, LDL-C） level change were measured by biochemical method, Serum endothelin （ ET-1 ） and nitric oxide （NO） level were detected by ELISA and ni- trate reductase method. In addition, HE staining was used to observe arterial morphological and Isolated vascular ring was used to test aortic endothelial diastolic function, while protein expression levels of PI3K, AKT and p-AKT in carotid tissue were detected by Western blot. Results Compared with the con- trol group, serum TC, TG, HDL-C, LDL-C, ET-1 level were increased （P 〈0.01 ） and NO level and aortic diastole rate were decreased （P 〈0.01 ） in the model group, while the relative expression levels of PI3K and p-AKT in carotid tissue were decreased （P 〈0.01 ）. Compared with the model group, serum TC, TG and LDL-C level were reduced in the high-dose Tongxinluo group and Atorvastatin group, and serum TG and LDL-C level were reduced in the medium-dose Tongxinluo group （P 〈0.05, P 〈0.01 ）. Serum ET-1 level were decreased and NO level and aortic diastole rate were increased （P 〈0.05, P 〈0.01 ） as well as the relative expression levels of PI3K and p-AKT in carotid tissue were increased （P 〈0.05, P 〈0.01 ） in the high-dose Tongxinluo group, medium-dose Tongxinluo group and Ato

关 键 词：高脂血症 通心络 内皮依赖性舒张功能 一氧化氮 P13K/AKT 

分 类 号：R285.5[医药卫生—中药学]