诊断剂量彩色多普勒超声对小鼠早孕胚胎组织细胞安全性的影响  

Effect of Diagnostic Dose of Color Doppler Ultrasound on Mouse Embryo Cells

作　　者：王锦惠[1] 刘立民[2] 魏濬 雷海胜[1] 贾胜斌 乔中东[2] 

机构地区：[1]太原市第二人民医院超声诊断科,太原市030002 [2]山西医科大学分子生物实验室 [3]太原市第一人民医院超声诊断科

出　　处：《中国超声医学杂志》2002年第8期578-581,共4页Chinese Journal of Ultrasound in Medicine

基　　金：太原市科委启明星计划资助

摘　　要：目的 :检测诊断剂量彩色多普勒超声不同时间照射后对早孕小鼠胚胎组织细胞凋亡及超微结构的影响。方法 :应用 3.5 MHz探头固定持续照射孕鼠腹部 ,2 4小时后取出标本 ,琼脂糖凝胶电泳观察不同照射时间的 DNA片段情况及细胞超微结构的变化。结果 :琼脂糖凝胶电泳显示 ,除对照组外 ,其余 3组均出现凋亡梯形 ,且随时间的延长 ,梯形越明显。电镜结果显示 ,除对照组外 ,其余各组均可见不同程度的凋亡特征改变 :细胞核变形 ,染色质稀疏 ;线粒体变形、肿胀、嵴模糊、消失、部分空泡化 ;内质网网腔扩张 ,核糖体部分脱落 ;凋亡小体形成。结论 :诊断超声持续照射小鼠胚胎可引起胚胎组织细胞凋亡及超微结构的变化 。Objective:To observe the effect of mouse embryo cell apoptosis and ultrastructure changes caused by diagnostic dose of color Doppler ultrasound.Methods:Early pregnant mice were irradiated with diagnostic dose of color Doppler ultrasound (3 5MHz). The DNA fragments were electrophoresed in an agarose gel and the fine structure changes were observed by electral microscope (EM).Results:Apoptic ladders were detected in the 3.5MHz transducer group except the control, as well as the ladder was clearer according to the treated time. Typical ultrastructure changes were seen in the same groups ,which showed rarefaction of nuclear chromosome, patches of condensed chromatin lying against the nuclear membrane, obscureness of swelling mitochondria, crista disappeared and vacuolized, apoptotic body appeared at the same time.Conclusions:Continuous irradiation by diagnostic dose of color Doppler ultrasound may result in mouse embryo cells apoptosis.

关 键 词：细胞凋亡 超微结构 诊断剂量 彩色多普勒超声 小鼠 早孕 胚胎组织 

分 类 号：R445.1[医药卫生—影像医学与核医学]