封闭群树鼩的微卫星遗传特性分析  

Analysis of Microsatellite Genetic Characteristics in of Closed Colony of Tree Shrews

作　　者：刘城秀 李娜 仝品芬 王文广 陆彩霞 罕园园 匡德宣 孙晓梅 代解杰 

机构地区：[1]中国医学科学院、北京协和医学院医学生物学研究所、云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《实验动物与比较医学》2018年第1期1-9,共9页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划项目(2014BAI01B00);云南省科技人才和平台计划项目(2017HC019);云南省重大科技专项(2017ZF007)

摘　　要：目的比较分析远交繁殖的I^IV代次的滇西亚种树鼩群体的遗传差异和分化程度,为培育封闭群树鼩种群提供遗传学依据。方法提取I^IV四个代次共49只滇西亚种树鼩的全血基因组DNA,选取树鼩的18个微卫星位点,结合荧光标记(FAM)PCR扩增技术,通过毛细管电泳技术检测扩增产物,并利用POPGENE、FSTAT等软件比较各代次树鼩的遗传相关指标。结果四个代次的树鼩群体共检测到等位基因(Na)89个,平均有效等位基因数(Ne)为3.779,平均观察杂合度(Ho)为0.523,平均期望杂合度(He)为0.613,平均多态信息含量(PIC)为0.558,平均Shannon信息指数(I)为1.277,平均等位基因丰富度(AR)为2.938,遗传分化系数(Fst)平均值为0.046;其遗传距离及无偏遗传距离分别为0.049~0.159和0.022~0.109。结论培育的树鼩群体的遗传多样性较丰富,且不存在较大的遗传差异和分化程度。Objective To find out the genetic variation and differentiation of the I-IV generations in tree shrews （Tupaia belangeri chinensis）, and provide genetic bases for closed colony tree shrews. Methods The forty-nine genomic DNA of blood samples were extracted from I-IV generations tree shrews, the amplification products were detected by capillary electrophoresis and marked with fluorescent tags （FAM） PCR, then the genetic diversity of the tree shrew colony was analyzed comparatively using POPGENE, FSTAT software with tree shrews 18 microsatellite loci. Results The 89 alleles were found in the tree shrew colony, the average effective number of alleles, observed heterozygosity, expected heterozygosity, polymorphism information content, Shannon information index and allele richness were 3.779, 0.523, 0.613, 0.558, 1.277 and 2.938 respectively. The mean value of Fst was 0.046 in the colony. The genetic distance and unbiased genetic distance were 0.049-0.159 and 0.022-0.109 respectively. Conclusions The closed clony tree shrew shows high genetic diversity, and there was no significant difference in genetic diversity and differentiation.

关 键 词：滇西亚种树鼩 封闭群 微卫星位点 遗传多样性 遗传管理 

分 类 号：Q95-33[生物学—动物学]