活性氧簇的小分子荧光探针研究进展  

Review of the Small Molecular Fluorescent Sensors for Intracellular Reactive Oxygen Species

作　　者：后际挺 李坤[3] 覃彩芹 余孝其[3] 

机构地区：[1]湖北工程学院生物质资源转化利用湖北省协同创新中心,孝感432000 [2]湖北工程学院化学与材料科学学院,孝感432000 [3]四川大学化学学院,成都610064

出　　处：《有机化学》2018年第3期612-628,共17页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.31371750)资助项目~~

摘　　要：在参与体内各种化学反应的生物活性分子中,活性氧(reactive oxygen species,ROS)是一类非常重要的物质,它们具有氧化性,能够维持细胞内的氧化还原平衡,与细胞的生长或凋亡息息相关.因此,对于细胞内活性氧簇的荧光分析一直以来都受到广大研究人员的密切关注.然而由于ROS本身自有的一些特性,如存在寿命短、反应活性高等,该类荧光分析始终面临着一些难题,如选择性较低、副反应较多.总结了近十几年来细胞中活性氧的荧光识别与检测的研究进展,着重介绍了各类荧光探针的设计机理与生物应用,并对未来该类探针的发展进行了展望.Among the numerous bio-active species which are involved in the various chemical reactions in our body, reactive oxygen species（ROS） are a class of important biological species, which are oxidative. ROS can maintain the intracellular redox balance and are tightly related with the cell growth and death. Hence, the fluorescence detection of intracellular ROS has attracted wide attention. However, owining to the inherent features of ROS, such as the short lifetime and high reactivity, fluorescence analysis of ROS is always faced with some problems, like low selectivity and side reactions. Herein, the development of small molecular fluorescent probes for intracellular ROS over the past decade is summarized, and the design mechanisms and bio-applications of these probes are emphasized.

关 键 词：活性氧簇 荧光探针 细胞成像 

分 类 号：O657.3[理学—分析化学]