新型1,8-萘二酰胺荧光探针的制备及其对Cu^(2+)检测应用  

Synthesis and application of new fluorescent probe based on 1,8-naphthalenediamide derivatives for the determination of Cu^(2+)

作　　者：李园芳 李海霞[1] 余光勤[1] 胡庆红[1] 袁泽利[1] 

机构地区：[1]遵义医学院药学院,遵义563003

出　　处：《分析试验室》2018年第3期280-285,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81360471;81660575);贵州省国际合作项目([2012]7036);贵州省卫生计生委科学技术基金项目(gzwjkj2015-1-001)资助

摘　　要：以4-溴-1,8-萘甲酸酐、水合肼、4-(2-氨乙基)吗啉和水杨醛作为原料,合成了一种1,8-萘二酰胺的新型荧光探针(L),并通过~1H NMR,^(13)C NMR,FT-IR等分析手段对其结构和组成进行表征。研究发现L对Cu^(2+)具有荧光识别作用且受其它离子干扰小的优势。探针L的荧光强度与Cu^(2+)浓度(2.0~9.0μmol/L)呈较好的线性关系,检出限为47 nmol/L。将其对河水样品和药品进行Cu^(2+)加标回收实验,回收率均大于92.0%,RSD小于2.0%,探针L能够应用于这些样品中微量Cu^(2+)的测定。A new 1,8-naphthalenediamide derivative L was synthesized by the condensation reaction from 4-bromo-1,8-naphthalimide,hydrazine hydrate and 4-（2-aminoethyl） morpholine and salicylaldehyde,and its structure was characterized by -1H NMR,-（13）C NMR,IR and HRMS-ESI spectra.The results showed that the probe L displayed high sensitivity toward Cu^2＋,while other ions exerted no interfere on its recognition.The fluorescence intensity of L showed a good linear relationship with Cu^2＋concentration from 2.0 to 9.0 μmol/L,and the detection limit is 47 nmol/L.Moreover,L has been applied to the determination of Cu^2＋in the actual water samples and drug sample.The result showed that the standard recoveries were more than 92.0% and the RSD values were within 2.0%,which further demonstrated that L is applicable for the quantitative determination of trace Cu^2＋in real samples.

关 键 词：荧光探针 1 8-萘二酰胺 席夫碱 铜(Ⅱ) 

分 类 号：R917[医药卫生—药物分析学]