成年大鼠皮下移植的幼年大鼠睾丸组织的发育和雄激素的分泌  

Subcutaneous transplants of juvenile rat testicular tissues continue to develop and secret androgen in adult rats

作　　者：余州[1] 王彤[1] 崔江波[1] 宋雅娟[1] 马显杰[1] 苏映军[1] 彭湃[1] 

机构地区：[1]第四军医大学西京医院整形外科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2017年第12期1652-1656,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30973752;81272118);西京医院学科助推计划(XJZT15M23)

摘　　要：目的探索成年大鼠皮下微环境对移植的幼年SD大鼠睾丸中睾丸间质细胞存活、发育及雄激素分泌的影响。方法将成年雄性SD大鼠随机分为对照组、假手术组、去势组和未去势组。对照组大鼠不进行任何处理;假手术组大鼠去势后不进行睾丸移植;去势组大鼠于去势1周后行背部双侧睾丸移植手术,每侧移植1个5~7 d SD大鼠睾丸组织;未去势组直接在正常大鼠背部两侧各移植1个幼年SD大鼠睾丸组织。移植后第4周取材、称量移植睾丸组织质量,HE染色分析移植睾丸组织的组织学特点,免疫组织化学染色法检测移植睾丸组织中17β-羟基类固醇脱氢酶1(HSD-17β1)表达和分布,同时采用ELISA检测受体血清睾酮水平。结果去势组移植睾丸组织质量明显大于未去势组。去势组和未去势组均可见睾丸间质细胞,免疫组织化学染色发现去势组移植睾丸组织中HSD-17β1阳性细胞较未去势组多。ELISA分析发现对照组和未去势组的血清雄激素水平均高于假手术组和去势组,且去势组明显高于假手术组。结论移植的幼年大鼠睾丸组织可在成年受体大鼠皮下进一步发育,形成的睾丸间质细胞还具备一定的雄激素合成和分泌功能。Objective To explore the effects of subcutaneous microenvironment of adult rats on survival,development and androgen secretion of Leydig cells of transplanted juvenile rat testis. Methods Healthy adult SD rats were randomly divided into control group,sham group,castrated group and non-castrated group. Rats in the control group were kept intact,no testis was transplanted subcutaneously after adult recipients were castrated in the sham group; 5-7-day juvenile rat testes were transplanted subcutaneously in the castrated group,with one testis per side; Testes resected from juvenile rats were directly transplanted subcutaneously on both sides of the recipients in the non-castrated group. The grafts were obtained and weighed 4 weeks later. Then the histological features of the grafts were examined by HE staining; the expression and distribution of hydroxysteroid 17-beta dehydrogenase 1（ HSD-17β1） were investigated by immunohistochemistry; and the serum androgen level was determined by ELISA. Results The average mass of grafts obtained from the castrated group was significantly higher than that of the non-castrated group. Immunohistochemistry indicated that Leydig cells were visible in the tissues from both the castrated and non-castrated groups,but the number of HSD-17β1-posotive cells in the castrated group was larger than that in the non-castrated group. ELISA results showed that the serum androgen level was higher in the control group and non-castrated group than in the sham group and castrated group,and compared with the sham group,the serum androgen level in the castrated group was significantly higher. Conclusion The juvenile rat testis subcutaneously transplanted could further develop under the adult recipient rat skin,and the Leydig cells of grafts harbored the ability to produce and secret androgen.

关 键 词：睾丸移植 睾丸间质细胞 雄激素 分泌 

分 类 号：R392-33[医药卫生—免疫学] R392.4[医药卫生—基础医学]