反相高效液相色谱法同时测定血清中苯妥英、卡马西平、苯巴比妥  

Simultaneous Determination of Phenytoin Carbamazepine and Phenobarbital in Serum by Reversed Phase High Performance Liquid Chromatography

作　　者：徐家明[1] 周爱华[1] 刘思忠[1] 王为民[2] 徐启刚[3] 郭菊英[3] 

机构地区：[1]甘肃省卫生厅临床检验中心,兰州730000 [2]兰州市兰州医学院第二附属医院 [3]甘肃省人民医院

出　　处：《中国医院药学杂志》1991年第5期195-197,共3页Chinese Journal of Hospital Pharmacy

摘　　要：本文报道一个专属、准确、精密、灵敏的抗癫痫药物苯妥英(Phenytoin DPH)、卡马西平(Carbamazepine,CBZ)和苯巴比妥(Phenobarbital,PB)血药浓度的反相高效液相色谱测定法。本法以5—乙基—5—对甲苯基巴比妥酸为内标,采用简单的溶液提取,于40℃、氮气流蒸干,流动相制成供试液进样。以乙腈、甲醇、水和氯仿(10∶35∶56∶0.13,V/V)为流动相,于254nm检测。在三个不同水平,DPH、CBZ、PB的平均回收率为97.81%、98.14%和100.7%(n=12),日内和日间变异均低于5%。内源性物质及34种常用药物未见干扰本测定条件,在分析范围内具有良好的线性关系,并已在临床分析中应用。A specific, accurate, precise and sensititive reversed-phase HPLC methodfor simultaneous determination of antiepileptic drugs. phenytoin(DPH). carbama-zepine (CBZ) and phenobarbital(PB), in serum with 254 nm detection and 5-ethyl-5-p-tolylbarbituric acid as internal standard (IS) was reported. DPH. CBZ. BPand IS were extracted from serum at pH 7.3 to methyltert-butyl ether, evaporatedat 40℃ under nitrogen gas, reconstituted with 50μ1 mobile phase, and injected ina 15×0.46 cm column which was packed with 10μ YWG C_(18)H_(37) .The mobile phaseof acetonitrile methanol-H_2O-chloroform (10: 35: 56: 0. 13, by vol) was used. Theaverage recovery at different levels for DPH, CBZ and PB were 97.81%. 98.14%and 100.7%(n=12)respectively. Endogenous compounds and 34 commonly used drugsdid not interfer with this chromatographic procedure. The analytical rallge waslinear from 19.8 to 158.5 μmo1/L for DPH, from 10.6 to 84.7μmol/L for CBZ andfrom 21.5 to 172.2μmol/L for PB.

关 键 词：抗癫痫药 血药浓度 高效液相色谱 

分 类 号：R969.1[医药卫生—药理学]