日粮纤维水平对鹅盲肠细菌多样性的影响  

Effects of Different Dietary Fiber Levels on Bacteria Diversity in Cecum of Goose

作　　者：周海柱[1] 高云航[1] 徐博[1] 陶大鹏 滕战伟 娄玉杰[1] ZHOU Hai-zhu, GAO Yun-hang, XU Bo, TAO Da-peng, TENG Zhan-wei, LOU Yu-jie(Animal Science and Technology College, Jilin Agricultural University, Changchun 130118, Chin)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《畜牧兽医学报》2018年第3期565-571,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2016YFD0501409-03);国家自然科学基金(31372331);现代农业产业技术体系建设专项(CARS-37);公益性行业(农业)科研专项(201303091)

摘　　要：旨在探讨不同纤维水平日粮对卡洛斯鹅盲肠细菌多样性的影响。选用35日龄健康卡洛斯公鹅90只,随机分成5%纤维水平组(L组)和8%纤维水平组(H组),每组3个重复,每个重复15只鹅,试验期为42d。试验结束后,采用高通量测序方法对鹅盲肠黏膜细菌进行分析。结果表明,H组检测出的细菌属于17门类的199个属,L组检测出的细菌属于19门类的226个属;H组和L组细菌多样性无显著差异(P>0.05);H组Verrucomicrobia、Deferribacteres菌门和Desulfovibrio、Prevotella、Helicobacter、Mucispirillum、Akkermansia菌属相对丰度显著高于L组(P<0.05),L组Synergistetes、Euryarchaeota菌门和Bacteroides、Methanobrevibacter菌属相对丰度显著高于H组(P<0.05)。结果提示,5%和8%日粮纤维水平对鹅盲肠细菌多样性无显著影响,但可显著影响Desulfovibrio、Prevotella、Helicobacter、Mucispirillum、Akkermansia、Bacteroides、Methanobrevibacter菌属的相对丰度。The objective of this study was to investigate the effects of different dietary fiber levels on cecum microflora diversity of Carlos geese.Ninety healthy Carlos male geese with 35 days old were divided into 2 groups（n=45）with 3 duplicates for 15 per duplicate,and then fed with diets containing 5%（L group）or 8%（H group）crude fiber using alfalfa as the crude fiber sources,respectively.After feeding 42 days,caecum microflora was determined using MiSeq PE300 sequencing platform.The results showed that caecum microflora of goose in H group included 17 phylums and 199 genera,while caecum microflora of goose in L group included 19 phylums and 226 genera.The bacteria diversity of each sample between the two groups was similar（P〈0.05）.The relative abundance of Verrucomicrobia,Deferribacteres,Desulfovibrio,Prevotella,Helicobacter,Mucispirillumand Akkermansia of H group significantly increased compared to L group（P〈0.05）;The relative abundance of Synergistetes,Euryarchaeota,Bacteroides and Methanobrevibacter of L group significantly increased compared to H group（P〈0.05）.These results suggest that there is no significant difference in the bacteria diversity of cecum of goose fed with5% or 8% crude fiber,however,the relative abundance of Desulfovibrio,Prevotella,Helicobacter,Mucispirillum,Akkermansia,Bacteroides,Methanobrevibacter are significantly different between the two groups.

关 键 词：纤维水平 鹅 盲肠 细菌 多样性 丰度 

分 类 号：S835.5[农业科学—畜牧学]