金银花花器发育时期与有效成分含量的关系  

The Relationship Between Floral Organ Development Period and Effective Component Content of Lonicera japonica

作　　者：崔旭盛[1,2] 马召[1,2] 田清存 高秀强 祁飞[1] 郭玉海[2] Cui Xusheng1＇2, Ma Zhao, Tian Qingcun, Gao Xiuqiang, Qi Fei, Guo Yuhai2(1. Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035; 2China Agricultural University, Beijing 10019)

机构地区：[1]石家庄以岭药业股份有限公司,石家庄050035 [2]中国农业大学,北京100193

出　　处：《中国农学通报》2018年第4期71-75,共5页Chinese Agricultural Science Bulletin

基　　金：国家中药标准化项目"中药大品种连花清瘟胶囊标准化研究"(ZYBZH-C-HEB-12);国家科技富民强县项目"优质树形金银花产业化技术集成与示范"(201305510110029)

摘　　要：为研究金银花花器发育时期与有效成分含量的关系,以幼蕾期、青蕾期、白蕾期、银花期、金花期5个不同发育时期的金银花样品为试验材料,采用高效液相色谱法(HPLC)法对绿原酸、咖啡酸、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C的含量、累积量及比例进行研究。结果表明:(1)绿原酸、咖啡酸、木犀草苷以及异绿原酸A含量在青蕾期最高,分别高达27.29、0.22、0.84、0.36 mg/g,而异绿原酸B、异绿原酸C和总异绿原酸含量峰值出现在幼蕾期,分别为7.22、1.77、9.30 mg/g。(2)绿原酸、咖啡酸、异绿原酸A、异绿原酸B、异绿原酸C和总异绿原酸累积量均在白蕾期最高,分别为82.19、4.12、5.56、101.67、23.05、130.07μg/朵,仅有木犀草苷累积量最大值出现在青蕾期(10.39μg/朵)。(3)随着金银花花器发育,绿原酸与木犀草苷比例先降低后增高,而异绿原酸A所占比例逐渐上升,异绿原酸B、C所占比例逐渐降低。金银花有效成分含量、累积量以及比例与发育时期密切相关,最适采收期为白蕾期。To study the relationship between floral organ development period and effective component content of Lonicera japonica, samples at five different developmental stages of L. japonica were collected and analyzed forthe content, accumulative amount and ratio of chlorogenic acid, caffeic acid, luteolin, isopropenic acid A, chloro-genic acid B and isochromatic acid C by HPLC method. The results showed that：（1） the contents of chlorogenicacid, caffeic acid, mignonette glycosides and isochlorogenic acid A were the highest in green bud period, respec-tively reached 27.29, 0.22, 0.84, 0.36 mg/g, but the peak content of isochlorogenic acid B, isochlorogenic acid Cand total isochlorogenic acid appeared in the infant stage, they were respectively 7.22, 1.77, 9.30 mg/g;（2） thecumulant of chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid Cand the total isochlorogenic acid were the highest in white bud period, they were respectively 82.19, 4.12, 5.56,101.67, 23.05, 130.07 μg/bloom; however the maximum cumulant of mignonette glycosides appeared in thegreen bud stage（10.39 μg/bloom）;（3） with the development of floral organ, the ratio of chlorogenic acid and mi-gnonette glycosides decreased first and then increased, the ratio of isochlorogenic acid A increased, while the ra-tio of isochlorogenic acid B and C reduced. In conclusion, the effective components＇ content, accumulationamount and proportion of L. japonica are closely related to its development period, and the optimal harvest time iswhite bud stage.

关 键 词：金银花 花期 有效成分 

分 类 号：S567[农业科学—中草药栽培]