RP-HPLC法同时测定乳痛丸中丹参酮类成分的含量  

Simultaneous determination of different kinds of tanshinone components in Rutong pills by RP-HPLC

作　　者：叶因涛[1,2] 钱钧强 吴春暖 胡利民 YE Yin-tao1,2, QIAN Jun-qiang2 , WU Chun-nuan2, HU Li-min1(1. Tianjin University of Traditional Chinese Medicine, Key Laboratory of Pharmacology of Traditiomal Chinese Medical Formulae,Ministry of Education, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacolo- gy, Tianjin 300193, China ; 2. Department of Pharmacy, Tianjin Medical University Cancer Institute and Hos- pital , National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China)

机构地区：[1]天津中医药大学中医药研究院,教育部方剂学重点实验室,天津市中药药理学重点实验室,天津300193 [2]天津医科大学肿瘤医院药学部,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津300060

出　　处：《时珍国医国药》2018年第2期308-311,共4页Lishizhen Medicine and Materia Medica Research

基　　金：天津市科委创新平台建设项目(16PTSYJC00120); 天津市卫计委中医中西医结合科研课题(2015066)

摘　　要：目的建立同时测定乳痛丸中丹参酮ⅡA、隐丹参酮和丹参酮I三种丹参酮类成分含量的方法。方法采用反相高效液相色谱法。色谱柱为Thermo-Syncronis C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-0.02%磷酸水溶液,梯度洗脱,流速为1.0 ml·min^(-1),检测波长为270 nm,柱温30℃,进样量10μl。结果丹参酮ⅡA、隐丹参酮和丹参酮I组分分离良好,线性范围合理(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率分别为99.12%(RSD=0.79%,n=9)、100.13%(RSD=0.51%,n=9)、98.62%(RSD=0.49%,n=9)。结论该方法结果准确、重复性好,可用于同时测定乳痛丸中的丹参酮ⅡA、隐丹参酮和丹参酮I含量。Objective To establish a method for simultaneous determination of three kinds of tanshinones including tanshinone ⅡA,cryptotanshinone and tanshinone I in Rutong pills.Methods RP-HPLC was performed on the column of Thermo-Syncronis C18（250 mm × 4.6 mm,5μm） with acetonitrle-0.02% phosphate solution as the mobile phase for gradient elution,the flow rate was 1.0 ml/min,detection wavelength was 270 nm,column temperature was 30 ℃ and the injection volume was 10 μl.Results The tanshinone components including tanshinone ⅡA,cryptotanshinone and tanshinone I had separated excellently with reasonable linear range（r = 0.999 9）; The RSD of precision,stability,reproducibility tests were lower than 2%; The average sample recovery rates were 99.12%（RSD = 0.79%,n = 9）,100.13%（RSD = 0.51%,n = 9） and 98.62%（RSD = 0.49%,n = 9）,respectively.Conclusion The method is accurate and reproducible,and can be used for the simultaneous determination of tanshinone ⅡA,cryptotanshinone and tanshinone I in Rutong pills.

关 键 词：反相高效液相色谱法 乳痛丸 丹参酮ⅡA 隐丹参酮 丹参酮I 含量测定 

分 类 号：R284.1[医药卫生—中药学]