硬粒小麦染色体的FISH核型分析  

FISH Karyotype Analysis of Chromosomes in Durum Wheat

作　　者：陈星灼 彭红 王亚[1] 杨婷[1] 彭泽 耿广东[1] 张庆勤[1,2] 张素勤 CHEN Xingzhuo1 , PENG Hong1 , WANG Ya1, YANG Ting1 , PENG Ze1, GENG Guangdong1 , ZHANG Qingqin1,2 , ZHANG Suqin1,2(1.College of Agriculture, Guizhou University, Guiyang 550025, China ; 2.Guizhou Subcenter of National Wheat Improvement Center, Guiyang 550025, Chin)

机构地区：[1]贵州大学农学院,贵阳550025 [2]国家小麦改良中心贵州分中心,贵州贵阳550025

出　　处：《种子》2018年第3期12-14,18,共4页Seed

基　　金：国家自然科学基金(31160224;31660390);国家"七大农作物育种"重点专项(2017YFD 0100900);贵州省农业成果转化计划项目(黔科合成果[2016]4022号);贵州大学自然科学专项科研基金(贵大专基合字[2013]01号)

摘　　要：采用FISH(荧光原位杂交)技术,分析硬粒小麦(Sauwne 20)染色体的FISH核型特点,为该种质在小麦新品种选育上的应用提供参考。结果表明,Sauwne 20包括14对染色体,Oligo-pTa 535-2红色探针信号主要分布在A组染色体上,而B组染色体上主要分布着明亮丰富Oligo-pSc 119.2-1绿色探针信号;根据这2种探针在Sauwne 20染色体上的分布特点,可以将其不同染色体进行准确地一一鉴别。Sauwne 20与中国春普通小麦的A组和B组染色体的FISH核型基本相似,但又有一定的差别,不同小麦材料间DNA重复序列表现出遗传多样性。In this experiment, FISH （Fluorescence in situ hybridization） technique was applied to analyze FISH patterns characteristics of chromosomes in durum wheat （Sauwne 20）, which would provide reference for the application of the germplasm in the breeding of new wheat varieties in the present study.The results showed that Sauwne 20 included 14 pairs of chromosomes,Oligo-pTa 535-2 red signals were mainly distributed on chromosomes of A genome,and the group B genome was main- ly distributed with bright and rich oligo-pscllg.2-1 green probe signals.According to the distribution characteristics of these two probes on Sauwne 20 chromosomes, different chromosomes can be accurately identified.Generally,Sauwne 20 is basically similar to the FISH karyotype of the chromosome A and B of Chinese spring wheat,but there is a certain difference, and the DNA replication sequence of different wheat materials shows genetic diversity.

关 键 词：硬粒小麦 FISH 核型 

分 类 号：S512.1[农业科学—作物学]