草莓根腐病的病原菌分离鉴定及拮抗菌CM3的抑制作用研究  

Isolation and Identification of Pathogenic Fungi of Strawberry Root Rot and the Inhibition of Antagonistic Bacteria CM3 on These Fungi

作　　者：陈哲[1] 黄静[1] 赵佳[1] 郝耀山[1] 梁宏[1] CHEN Zhe ,HUANG Jing ,ZHAO Jia ,HAO Yao-shan ,LIANG Hong(Biotechnology Research Center, Shanxi Academy of Agriculture Science, Taiyuan 030031)

机构地区：[1]山西省农业科学院生物技术研究中心,太原030031

出　　处：《生物技术通报》2018年第2期135-141,共7页Biotechnology Bulletin

基　　金：山西省农业科学院特色农业技术攻关(YGG17067);山西省应用基础研究项目(201601D202062)

摘　　要：从草莓根腐病病株根部成功分离了2株真菌FC2016-3和FC2016-7,经过鉴定确定其为根腐病的病原菌——尖孢镰刀菌(Fusarium oxysporum)。解淀粉芽孢杆菌(Bacillus amyloliquefacien)CM3对菌株FC2016-3和FC2016-7有明显的抑制作用,也对其他土传病害病原菌有较好的抑制效果。盆栽实验结果显示,菌株CM3对尖孢镰刀菌FC2016-7引起的草莓根腐病的防治效果达到了64.86%,而且还具有促进草莓根部生长的能力。研究表明菌株CM3是一株既能防病也能促生的菌株,适合开发成微生物制剂。In this paper,two fungi FC2016-3 and FC2016-7 were successfully isolated from the diseased root of strawberry,andidentified as Fusarium oxysporum. Bacillus amyloliquefacien CM3 presented obvious inhibitory effect on FC2016-3 and FC2016-7,and alsohad a promising inhibitory effect on other soil borne pathogens. The results of pot experiment showed that the control effect of strain CM3 onstrawberry root rot caused by F. oxysporum FC2016-7 was up to 64.86%,and strain CM3 also demonstrated the ability of promoting rootgrowth of strawberry. This study reveals that strain CM3 is a strain that can prevent soil borne disease and simultaneously promote plant growth,therefore,it is appropriate for the development of microbial preparation.

关 键 词：尖孢镰刀菌 解淀粉芽孢杆菌 生物防治 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]