基于大鼠体内多成分代谢的黄芪质控成分遴选  

Selection of quality control components from Astragali Radix based on multicomponent metabolism in rats

作　　者：刘傲雪 王晶娟[1] 张贵君[1] 刘洋[1] 鲁利娜[1] 王月[1] 张久旭 李小阳 马晓云[1] Liu Aoxue1, Wang Jingjuan1, Zhang Guijun1, Liu Yang1, Lu Lina1, Wang Yue1, Zhang Jiuxul, Li Xiaoyangl, Ma Xiaoyun1(Beijing University of Chinese Medicine, Beijing 10248)

机构地区：[1]北京中医药大学,北京102488

出　　处：《药物评价研究》2018年第2期216-222,共7页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(81303217)

摘　　要：目的基于中药多成分作用特点,探讨黄芪多成分在体内的代谢情况,遴选黄芪质控成分。方法制备黄芪水提物,ig给予大鼠(生药20 g/kg),每天3次,连续给药3 d,腹主动脉采血、代谢笼收集尿液,结合超高效液相色谱-串联质谱(UPLC-MS/MS)技术对黄芪水提物主要成分、入血成分和尿液排泄成分进行鉴别,并分析主要的代谢途径,遴选黄芪质控成分。结果黄芪水提物主成分共鉴别出包括毛蕊异黄酮葡萄糖苷、芒柄花苷、calycosin-7-O-glc-6"-O-malonate、毛蕊异黄酮、芒柄花素等在内的15个化合物;血浆样品中指认出11个成分,包括7个代谢成分和4个原型成分;尿液样品中指认出14个成分,9个代谢成分及5个原型成分。结论根据质谱数据分析结果,遴选出黄芪中以原型入血的成分和参与体内代谢的成分,包括毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、大豆苷元、3-hydro-9,10-di MP、isomer of 3-hydro-9,10-di MP、calycosin-7-O-glc-6"-O-acetate和formononetin-7-O-glc-6"-O-acetate,作为黄芪的质控成分。Objective To investigate the internal metabolism of Astragali Radixin and to select the quality control components of Astragali Radix by using UPLC-MS/MS based on the feature of multi-component.Methods Water extract of Astragali Radix was prepared for ig administration（crude drug 20 g/kg） to rats for 3 d,three times a day.The metabolic components in the rats blood from abdominal aorta and urine collected by metabolic cage,as well as water extracts after oral administration were analyzed by UPLC-MS/MS with view to analyzing the metabolic pathway and the quality control components of Astragali Radix.Results There are 15 known constituents identified in the decoction of Astragali Radix,including calycosin-7-O-β-D-glucoside,ononin,calycosin-7-O-glc-6＂-O-malonate,calycosin,formononetin etc.Eleven constituents were detected in the blood,including seven metabolic components,and four prototypes.There are 14 constituents identified in the Urine sample,including nine metabolic components and five prototypes.Conclusion There 10 components metabolic components and prototypes as quality control ingredients in water extracts of A.Radix based on the results of MS,which included calycosin-7-O-glucoside,ononin,calycosin,formononetin,daidzein,3-hydro-9,10-di MP,isomer of 3-hydro-9,10-di MP,calycosin-7-O-glc-6＂-O-acetate and formononetin-7-O-glc-6＂-O-acetate.

关 键 词：黄芪 水提物 多成分代谢 遴选 质控成分 血液 尿液 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：R965[医药卫生—药理学]