地鳖虫HPLC指纹图谱研究  

HPLC fingerprint of traditional Chinese medicinal insect Eupolyphaga sinensis Walker

作　　者：孙龙[1,2] 何钊[1,2] 赵敏[1,2] 和锐[1,2] 冯颖 SUN Long, HE Zhao, ZHAO Min, HE Rui, FENG Ying(Research Institute of Resources Insects, CAF; Key Laboratory of Cultivation and Utilization of Resource Insects, Sate Forestry Administration, Kunming 650233, Chin)

机构地区：[1]中国林业科学研究院资源昆虫研究所 [2]国家林业局资源昆虫培育与利用重点实验室,昆明650233

出　　处：《环境昆虫学报》2018年第1期23-29,共7页Journal of Environmental Entomology

基　　金：国家林业局"西南特色昆虫资源及经济树种种质资源收集保存与良种基地建设"项目;中央级公益性科研院所基本科研业务费专项(riricaf2014002Z)

摘　　要：地鳖虫Eupolyphaga sinensis Walker系我国传统的活血化瘀虫药之一,应用历史久远、疗效确切,但是其质量控制长期处于主观经验水平。为了建立地鳖虫指纹图谱,本研究采用高效液相色谱法(Agilent ZORBAX Eclipse XDB-C18色谱柱,150×4.6 mm,5μm),以甲醇-水(含0.05%三氟乙酸)为流动相梯度洗脱,检测波长254 nm,柱温35℃,流速1 m L/min,上样量5μL,通过"中药色谱指纹图谱相似度评价系统(2012 130723版)"软件对10批不同来源的地鳖虫进行相似度评价,筛选指纹特征峰。结果 10批不同来源的地鳖虫与对照指纹图谱的相似度为0.900-0.995,说明样品有较高的一致性,质量较好,确定了18个共有峰作为指纹峰。该指纹图谱建立方法简便、可靠,可定性用于地鳖虫药材的质量控制。Eupolyphaga sinensis Walker（E.sinensis） is one of the most important traditional medicinal insects with definite effect on promoting blood circulation and removing blood stasis and has been used in clinical applications from ancient times in China.Its quality control,however,is always at the level of subjective experience.In our study,high performance liquid chromatography（HPLC） with diode array detector was employed to establish the fingerprint of E.sinensis.Totally ten batches of samples was eluted on an Agilent ZORBAX Eclipse XDB-C18 column（150 × 4.6 mm,5 μm） using a mixture of methanol and water containing 0.05% trifluoroacetic acid（TFA） as mobile phase with linear gradient elution at a flow rate of 1.0 m L/min.The detection wavelength was 254 nm.The column temperature was 35℃,and the injection sample volume was 5 μL.The data was analyzed by the software ＂The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（2012 130723 Version） ＂from Chinese Pharmacopoeia Commission.The HPLC fingerprint of E.sinensis was achieved and the similar degrees of the 10 batches of samples were from 0.900 to 0.995,which indicated that the similarities of samples were high and their quality were good.18 common chromatographic peaks were chosen as characteristic peaks.The HPLC fingerprint of E.sinensis is simple and reliable and can beuseful for its quality control and evaluation.

关 键 词：地鳖虫 高效液相色谱 指纹图谱 共有峰 相似度 

分 类 号：Q963[生物学—昆虫学] S899.4[农业科学—特种经济动物饲养]