HPLC-UV法同时测定葫芦素片中葫芦素B和葫芦素E的含量  

Simultaneous determination of cucurbitacin B and cucurbitacin E in cucurbitacin tablets by HPLC-UV

作　　者：张雅男[1] 高明洁[2] 祝文博[1] 王知斌[1] ZHANG Ya-nan1, GAO Ming-jie2,ZHU Wen-bo1, WANG Zhi-bin11(1.Key Laboratory of Chinese Materia Medica（Ministry of Education）, Heilongjan$ Key Laboratory of TCM Pharmacodynamic Material Bases, Heilongjiang University of Chinese Medicine, Harbin 150040, China; 2.College of Pharmacy, Harbin Medical University,Harbin 150081,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室/黑龙江省中药天然药物药效物质基础重点实验室,黑龙江哈尔滨150040 [2]哈尔滨医科大学药学院,黑龙江哈尔滨150081

出　　处：《化学工程师》2018年第3期18-20,共3页Chemical Engineer

基　　金：博士后研究人员落户黑龙江科研启动资助金项目(LBHQ16214);黑龙江中医药大学"优秀创新人才支持计划"科研项目(2012RCD05);黑龙江中医药大学"杰出人才培育"基金项目(2012cj02)

摘　　要：目的:建立反相高效液相紫外法同时测定葫芦素片剂中葫芦素B、葫芦素E的含量测定方法。方法:采用Diamasil TM C_(18)柱(250×4.6mm,5μm),以0.1%甲酸水-乙腈为流动相,流速为1.0m L·min^(-1),检测波长为254 nm,柱温为35℃。结果:在40 min内,葫芦素片中葫芦素B(Cu B)和葫芦素E(Cu E)完全分离,进样的质量浓度与色谱峰面积呈现良好的线性关系,Cu B及Cu E的线性方程分别为:Y=8998X-18.38(r=0.9995),Y=10960X-73.14(r=0.9999),Cu B及Cu E的平均加样回收率分别为97.34%(RSD=2.71%)、96.68%(RSD=2.33%)。结论:该方法操作简便灵敏、结果准确可靠,样品回收率高,适用于片剂中葫芦素B和葫芦素E的含量测定。Objective： To establish a method for the simultaneous determination of cucurbitacin B （CUB） and cucurbitacin E（CuE） in cucurbitacin tablets by reversed-phase high performance liquid chromatography.Methods： The Diamasil TM C18 column （250×4.6mm,5μm） was used for separated. The mobile phase consisted of 0.1% aqueous formic acid - acetonitrile,the flow rate was 1.0mL＇min-l.The detection wavelength was 254 nm and the column temperature was 35℃.Resuhs：The cucurbitacin B and cucurbitacin E were completely separated in the cucurbitacin tablet within 40 min. The linearity of the CuB and CuE was linear with the mass concentration of the cucurbitacin and the peak area of the chromatogram. The linear equations of CuB and CuE were Y= 8998X-18.38（r= 0.9995） and Y=10960X-73.14 （r=0.9999） respectively. The average recoveries of CuB and CuE were 97.34% （RSD=2.71%）, 96.68% （RSD = 2.33%） .Conclusion ： The method is simple and sensitive, and the results are accurate and reliable.The sample recovery rate is high, This method is suitable for the determination of cucurbitacin B and cucurbitacin E in tablets.

关 键 词：高效液相色谱 葫芦素片 含量测定 

分 类 号：R917[医药卫生—药物分析学]