巴尔通体对19种抗生素体外敏感性分析  

In vitro susceptibility of Bartonella to 19 antibiotics

作　　者：宋秀平[1] 栗冬梅[1] 孙继民[2] 黄儒婷[3] 刘起勇[1] Song Xiuping1, Li Dongmei1, Sun Jimin2, Huang Ruting3, Liu Qiyong1(1. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute .for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, China; 3. Fengtai District Center for Disease Control and Prevention, Beijing 100071, Chin)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,世界卫生组织媒介生物监测与管理合作中心,北京102206 [2]浙江省疾病预防控制中心,浙江杭州310051 [3]北京市丰台区疾病预防控制中心,北京100071

出　　处：《疾病监测》2018年第1期26-31,共6页Disease Surveillance

基　　金：国家自然科学基金(No.81141108);国家科技重大专项(No.2017ZX10303404007)~~

摘　　要：目的检测巴尔通体菌株对10类19种抗生素最低抑菌浓度(MICs),分析药物敏感性及耐药性,为指导临床用药和耐药监测提供实验和数据参考。方法采用E试验法,检测巴尔通体属11种、35株菌株对强力霉素、阿奇霉素、利福平等19种抗生素的MICs。将培养的巴尔通体菌制成McFarland(MCF)2.0浊度的菌悬液,均匀涂布于含5%去纤维羊血的胰酶大豆琼脂培养基上,置5%CO2的37℃培养箱培养。培养5~7 d后判读MICs。结果 35株巴尔通体菌在体外对强力霉素、阿奇霉素、红霉素、克拉仙霉素等13种抗生素敏感,MICs≤0.016 mg/L;34株对利福平敏感,MICs≤0.002 mg/L;对克林霉素、丁胺卡那霉素、万古霉素、多粘菌素和磺胺类5种抗生素不敏感,MICs较高。结论绝大多数巴尔通体菌对强力霉素等14种抗生素敏感,但也对克林霉素等5种抗生素不敏感,在对巴尔通体病进行治疗时要注意选择敏感药物。Objective To detect the minimum inhibitory concentrations （MICs） of 19 antibiotics in 10 categories to Bartonella strains, analyze the drug susceptibility of Bartonella and provide evidence for clinical antibiotic use and drug resistance monitoring. Methods The susceptibility of Bartonella strains to 19 antibiotics and the antibiotic MICs were detected by using E test, the MICs of 19 antibiotics, including doxycycline, azithromycin and rifampicin, to Bartonella were detected by using 35 strains of strains of Bartonella in 11 genera. The McFarland bacterium suspension with turbidity 2.0 was made by using cultured Bartonella strains and coated evenly on trypsin soybean agar medium containing 5% defibrinated sheep blood. The medium was placed in incubator of 5% CO2 at 37 ~C for 5 - 7 days to detect MICs. Results Thirty five strains were sensitive to 13 antibiotics, including doxycycline, azithromycin, erythrocin and clarithromycin, the MICs were ~〈0.016 mg/L. Thirty four strains were sensitive to rifampicin, the MIC was ~〈 0.002 mg/L. They were not sensitive to clindamycin, amikacin, vancomycin, polymixin, and trimethoprim-sulfamethoxazole. The MICs of these antibiotics were significantly higher. Conclusion Most Bartonella strains were sensitive to 14 antibiotics, including doxycycline, but they were not sensitive to 5 antibiotics, including clindamycin. It is necessary to select antibiotics to which Bartonella is sensitive in the treatment of bartonellosis.

关 键 词：E试验 最低抑菌浓度 抗生素 巴尔通体 

分 类 号：R373[医药卫生—病原生物学]