Fluorescence Responses of the Protonation and Deprotonation Processes between Phenolate and Phenol within Rosamine  

作　　者：Ling Yang Jinyun Niu Yanhua Zhan Yujie Xu Ru Sun Jianfeng Ge 

机构地区：[1]College of Chemistry, Chemical Engineering and Material Science, Soochow University, 199 Ren'Ai Road, Suzhou, Jiangsu 215123, China [2]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, China [3]Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou215163, China

出　　处：《Chinese Journal of Chemistry》2018年第1期42-46,共5页中国化学（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China （51273136）, Natural Science Fund of Jiangsu Province （BK20151262）, a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions （PAPD）, the Project of Scientific and Technologic Infrastructure of Suzhou （SZS201708） and Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions.

摘　　要：Two rosamine-based pH probes 1a and 1b with pyronine-phenol skeleton were designed and synthesized by a simple one-step reaction, pH titration experiments showed that probes 1a and 1b exhibit near OFF-ON fluorescence responses around 550--750 nm towards the hydrogen ions. The pKa of the probe 1a is 8.29, while that of the probe lb increases to 12.1 because of the hydrogen bond inside it. Selective and competitive experiments indicated that both common ions and amino acids did not interfere their emission with hydrogen ions. Moreover, confocal fluorescent imaging showed that the probe la could be served as mitochondria biomarker in HeLa and Ges-1 cells.Two rosamine-based pH probes 1a and 1b with pyronine-phenol skeleton were designed and synthesized by a simple one-step reaction, pH titration experiments showed that probes 1a and 1b exhibit near OFF-ON fluorescence responses around 550--750 nm towards the hydrogen ions. The pKa of the probe 1a is 8.29, while that of the probe lb increases to 12.1 because of the hydrogen bond inside it. Selective and competitive experiments indicated that both common ions and amino acids did not interfere their emission with hydrogen ions. Moreover, confocal fluorescent imaging showed that the probe la could be served as mitochondria biomarker in HeLa and Ges-1 cells.

关 键 词：rosamine phenolate and phenol protonation and deprotonation pH probe mitochondria biomarker 

分 类 号：Q253[生物学—细胞生物学] TM923.321[电气工程—电力电子与电力传动]