双光子荧光寿命成像在肿瘤诊断研究中的应用  

Applications of Two-Photon Excitation Fluorescence Lifetime Imaging in Tumor Diagnosis

作　　者：李慧[1] 夏先园 陈廷爱 余佳 李曦[2] 郑炜[1] Li Hui1, Xia Xianyuan1, Chen Tingai1, Yu Jia1, Li Xi2, Zheng Wei1(1Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China ; 2Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, Chin)

机构地区：[1]中国科学院深圳先进技术研究院生物医学光学与分子影像研究室,广东深圳518055 [2]北京大学深圳医院消化内科,广东深圳518036

出　　处：《中国激光》2018年第2期133-146,共14页Chinese Journal of Lasers

基　　金：国家自然科学基金(81471702;81701744);国家重点研发计划(2017YFC0110200);中国博士后科学基金资助项目(2017M612763);广东省自然科学基金博士启动项目(2017A030310308);深圳市科技计划项目基础研究(JCYJ20150403091443326;YJ20150521144321005;JCYJ20150401145529037;JCYJ20160608214524052);SIAT优秀青年创新基金(2016020)

摘　　要：双光子荧光寿命成像技术在对生物组织进行高分辨三维成像的同时能获得生物组织的生化特性信息,实现结构和功能的精细定量表征,为生物组织的非侵入、无标记、活体成像提供了一种强有力的工具。该技术在肿瘤检测方面具有广阔的临床应用前景,已成为当前生物医学领域研究的热点之一。首先简要介绍了双光子荧光寿命的概念和常用检测方法;其次,结合课题组开展的胃癌和神经胶质瘤诊断等相关研究成果,详细介绍了双光子荧光寿命成像技术在消化道肿瘤、脑肿瘤及皮肤癌等肿瘤检测方面的最新研究进展;最后展望了该技术在未来临床应用中的潜在优势和可能面临的挑战。Two-photon excitation fluorescence lifetime imaging is an ingenious technique which can provide both morphological and biochemical information of tissues in three-dimensional space with subcellular resolution.The structural and functional properties of tissue can be quantitatively characterized in detail.Therefore,this technique provides a powerful tool for the noninvasive,label-free,intravital imaging of biological tissues,and which is promising to be applied in clinical diagnosis of tumors.In recent years,this technology has been used to detect tumors,and which has already become one of the research foci in the field of biomedicine.In this review paper,firstly,the concept of the two-photon excitation fluorescence lifetime and common fluorescence lifetime detection methods are introduced simply;secondly,we summarize the recent progresses of using two-photon excitation fluorescence lifetime imaging to detect various tumors,including digestive tract tumors,brain tumors,skin tumors,and so on,and among them,our results on the detection of gastric cancers and glioma are elaborately depicted;Finally,the potential advantages and possible challenges of this technology in future clinical application are provided and discussed.

关 键 词：生物光学 双光子显微成像 荧光寿命成像 肿瘤诊断 自发荧光 

分 类 号：O437[机械工程—光学工程]