基于sCMOS相机的超分辨定位成像技术  

Super-Resolution Localization Microscopy with Scientific Complementary Metal Oxide Semiconductor Camera

作　　者：桂丹[1,2,3] 商明涛 黄振立[1,2] Gui Dan1,2,3, Shang Mingtao1,2, Huang Zhenli1,2(1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China ; 2 Ministry of Education Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China ; a School of Electronic Engineering, Wuhan Vocational College of Software and Engineering, Wuhan, Hubei 430205, Chin)

机构地区：[1]华中科技大学-武汉光电国家实验室(筹)、Britton Chance生物医学光子学研究中心,湖北武汉430074 [2]华中科技大学工程科学学院、生物医学光子学教育部重点实验室、生物医学工程协同创新中心,湖北武汉430074 [3]武汉软件工程职业学院电子工程学院,湖北武汉430205

出　　处：《中国激光》2018年第2期200-207,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(81427801,61421064); 国家973项目(2015CB352003)

摘　　要：超分辨定位成像是一种代表性的超分辨成像技术,弱光探测器是该技术不可或缺的组成部分。和传统的串行输出EMCCD相机相比,并行输出sCMOS相机具备成像视场大、成像速度快和读出噪声低等优点,为超分辨定位成像带来了新的机遇,可在视频速率成像和大视场成像中取得明显成效。基于sCMOS相机的超分辨定位成像技术面临着高速相机带来的海量数据,需要解决数据传输、存储和计算等多环节的问题。从超分辨成像技术及相机的发展着手,讨论了基于sCMOS相机的超分辨定位成像技术的发展现状以及面临的机遇与挑战。Super-resolution localization microscopy（SRLM）is a representative super-resolution imaging technology.Low-light detector is an essential component in SRLM.Compared with traditional electron multiplying charge coupled device（EMCCD）cameras using serial output,scientific complementary metal oxide semiconductor（sCMOS）cameras with parallel output provide simultaneously large field of view,high imaging speed and low read noise,thus bring new opportunities for SRLM,especially on achieving video-rate imaging and large field of view imaging.At the same time,SRLM based on sCMOS camera is also facing several challenges which are mainly originated from the high-speed camera,including but not limited to massive data transmission,storage,and analysis.This paper introduces the principle and development of SRLM,and discusses the current status,opportunities and challenges of SRLM based on sCMOS camera.

关 键 词：生物光学 超分辨成像 sCMOS相机 定位成像 大视场 

分 类 号：TH742[机械工程—光学工程]