基于双点光学参数监测的生物组织激光热毁损评估  

Laser Thermal Damage Evaluation of Biological Tissues Based on Monitoring of Dual-Point Optical Parameters

作　　者：贾威威[1] 戴丽娟[1] 花国然[1] 钱志余[2] Jia Weiwei1, Dai Lijuan1, Hua Guoran1, Qian Zhiyu2(1School of Mechanical Engineering, Nantong University, Nantong, Jiangsu 226019, China ; 2Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics Nanjing, Jiangsu 210016, Chin)

机构地区：[1]南通大学机械工程学院,江苏南通226019 [2]南京航空航天大学生物医学工程系,江苏南京210016

出　　处：《中国激光》2018年第2期295-299,共5页Chinese Journal of Lasers

基　　金：国家自然科学青年基金(61405098)

摘　　要：以波长为720nm处的散射光强I_(720)为评估光学参数,探讨了激光热毁损过程中用远中心处双点光学参数实时评估近中心处热毁损效果的可行性。首先,对离体猪肝进行激光热毁损实验,实时采集距离毁损中心4,8,12mm处的I_(720),当8mm处的I_(720)上升至初值的6倍时停止加热,共进行20组实验(A组),构建加热时间、8mm和12mm处I_(720)上升倍数与4mm处I_(720)上升倍数的数学模型;然后,分别以4 mm处I_(720)上升至初值的3、4、5、6倍为停止加热的条件,重复上述热毁损实验和实时数据采集,每种条件下各进行10组实验,共40组(B组);最后,对B组实验的样本进行切片分析,建立I_(720)上升倍数与毁损效果的对应关系。实验表明,利用加热时间、8 mm和12mm处I_(720)上升倍数估算4mm处I_(720)上升倍数的准确率可达90%以上。可用远离加热中心的双点光学参数实时评估近中心处激光热毁损效果。The scattering intensity at 720 nm（I720is chosen as the optical parameter of evaluation,and we use the dual point optical parameters far from the center to evaluate the thermal damage near the center during the process of laser thermal damage.Firstly,we test on the laser thermal damage of in vitro porcine livers to collect I720 in real time at points which are 4,8,12 mm far from the heating center,and laser heating is stopped when I720 at 8 mm rises to 6 times compared with its initial value（group A）.We conduct twenty experiments to calculate rising multiple of I720 at 4 mm far from the center with the mathematical model using the parameters of heating time,rising multiples of I720 at 8 mm and 12 mm.Secondly,we repeat the above thermal damage experiments and realtime data acquisition when the condition of stopping heating is I720 at 4 mm rising to 3,4,5,6 times（group B）.Ten experiments are done for each condition,and forty experiments are established.Finally,the slice analysis on samples of group B are taken to establish the relationship between the rising multiples of I720 and the damage effect.The results show that the accuracy is above 90% when heating time and rising multiples of I720 at 8 mm and 12 mm are used to estimate rising multiple of I720 at 4 mm.It is feasible to evaluate the effect of laser thermal damage in real time at a point near the damage center by dual-point optical parameters far from the center.

关 键 词：医用光学 光谱分析 激光生物学 热毁损 实时评估 

分 类 号：O433.4[机械工程—光学工程]