甘草次酸对心肌缺血再灌注损伤的影响  

Protective Effect of Glycyrrhetinic Acid on Myocardial Ischemia/Reperfusion Injury

作　　者：金敏 吴红金 JIN Min;WU Hong-jin(Beijing Hospital of Integrated Traditional Chinese and Western Medicine, Beijing 100039, China)

机构地区：[1]北京市中西医结合医院,北京100039

出　　处：《中国药学杂志》2018年第5期359-363,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30873234);北京中医药重点学科项目(京中重312)

摘　　要：目的观察甘草次酸(glycyrrhetinic acid)对大鼠实验性心肌缺血再灌注损伤的影响。方法用结扎冠状动脉左前降支近端的方法建立心肌缺血大鼠模型,通过结扎30 min再灌180 min观察大鼠血流动力学的变化、心肌梗死面积,血清CK-MB、LDH的情况。结果甘草次酸高、中、低剂量组心肌缺血30 min再灌注180 min期间,各组心率(HR)、平均动脉压(MAP)和血压心率指数(PRI)较模型组差异不显著,无统计学意义(P>0.05);甘草次酸高剂量组、中剂量组心肌梗死面积较模型组明显降低(P<0.05)。甘草次酸各组心肌缺血再灌注末CK-MB含量较模型组明显降低(P<0.05);甘草次酸高、中剂量组LDH含量与模型组相比有所降低,差异有显著性意义(P<0.05)。结论甘草次酸可对大鼠心肌缺血再灌注损伤有保护作用。OBJECTIVE To explore the protective effect of glycyrrhetinic acid （GA） on myocardial ischemia/reperfusion injury. METHODS Forty-eight healthy male SD rats were randomly and averagely divided into six groups： sham, control, and control with tanshinone II A sodium injection, GA at low, median and high dosages. The rats were subjected to 30 rain of ischemia, followed by 180 min reperfusion. The influence of GA on arrhythmic during reperfusion period was observed. At the end of reperfusion period, the concentrations of CK-MB and LDH in serum were measured with assay kits, and the myocardial infarct size was measured with colour- ing method. RESULTS At the time of myocardial ischemia and reperfusion, the HR, MAP and PRI in any GA group were not signif- icantly different compared with the control group. At the end of the reperfusion period, the myocardial infarct sizes were decreased sig- nificantly in median and high dosage GA groups compared with the control group （P 〈 0. 05） ; the CK-MB concentration in serum in all the GA groups were much lower （ P 〈 0.05 ） ; the LDH concentrations in serum were much lower in median and high dosage GA groups （P 〈 0. 05 ）. CONCLUSION GA exerts protection against myocardial ischemia/reperfusion as shown by a significant reduction in infarct size and decreased concentrations of CK-MB and LDH in serum.

关 键 词：甘草次酸 心肌缺血再灌注 心肌保护 

分 类 号：R965[医药卫生—药理学]