大孔吸附树脂富集纯化罗汉果茎叶中山奈苷的工艺研究  

Purification process of Kaempferol 3,7-O-L-dirhamnoside from Siraitia grosvenorii stem and leaf by macroporous resin

作　　者：仲冉 何珺[1,2] 徐洋[1] 陈震[2] 王黔阳 ZHONG Ran1, HE Jun1,2, XU Yang1, CHEN Zhen2, WANG Qian-yang2(1.College of Pharmacy, Guizhou University, Guiyang 550025; 2.Research Center of Biochemistry Engineering of Guizhou Province, Guiyang 55002)

机构地区：[1]贵州大学药学院,贵阳550025 [2]贵州省生化工程中心,贵阳550025

出　　处：《食品科技》2018年第3期196-200,共5页Food Science and Technology

基　　金：贵州大学研究生创新基金项目(研农2017029;研农2017030;研农2011017);贵阳市科技计划项目(2012204)

摘　　要：目的:探究大孔吸附树脂富集纯化罗汉果茎叶中山奈苷的最佳工艺。方法:以废弃罗汉果茎叶为原料,采用高效液相测定山奈苷含量,以吸附率和解吸率为指标,采用静态吸附试验对5种大孔树脂进行筛选,优选出吸附解吸性能最佳的大孔树脂,并对纯化条件进行优化,确定最佳工艺参数。结果:AB-8型树脂对罗汉果茎叶中山奈苷有较好的吸附和解吸附效果,其工艺为:上样液质量浓度0.8019 mg/mL,吸附流速为2 BV/h,上柱量为13 BV,以5 BV 50%的乙醇洗脱,洗脱流速为2 BV/h,纯化后产品中山奈苷的纯度高达52.12%。结论:AB-8型树脂适合富集纯化罗汉果茎叶中山奈苷。Objective： A method for purification of Kaempferol 3,7-O-L-dirhamnoside from Siraitia grosvenorii stem and leaf with macroporous resin was studied. Methods： The content of Kaempferol 3,7-O-L-dirhamnoside was determined by the high performance liquid chromatography （HPLC）. By using adsorption rate and desorption rate as the indices, five kinds of macroporous resins were screened by the static adsorption test. Preferably the adsorption and desorption properties of the best macroporous resin, and the optimal purification conditions of resin were obtained based on dynamic adsorption and static adsorption. Results： Experiment results showed that AB-8 resin possessed high absorption capacity. The best technical parameters was the content of sample solution 0.8019 mg/mL, the velocity of flow 2 BV/h, the maximum adsorbing capacity 13 BV, the elution rate 2 BV/h and 5 BV of 50% ethanol eluting solvent. The purity of Kaempferol 3,7-O-L-dirhamnoside was measured to be 52.12%. Conclusion： The AB-8 macroporous adsorption resin has the best enrichment and purification efficiency for Kaempferol 3,7-O-L- dirhamnoside from Siraitia grosvenorii stem and leaf.

关 键 词：罗汉果茎叶 山奈苷 纯化 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]