HPLC梯度洗脱联合波长切换法测定龟鹿补肾胶囊中7种成分的含量  

Determination of seven components in Guilu Bushen capsule by HPLC gradient elution combined with wavelength switching method

作　　者：席艳[1] XI Yan(Department of Pharmacy, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China)

机构地区：[1]大连医科大学附属第一医院药学部,辽宁大连116011

出　　处：《沈阳药科大学学报》2018年第3期207-212,共6页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立HPLC法同时测定龟鹿补肾胶囊中的7种成分。方法采用Alltima C18色谱柱;以乙腈-体积分数0.2%磷酸溶液为流动相,梯度洗脱;切换波长检测,检测波长分别为330 nm(麦角甾苷、吉奥诺苷B1)和270 nm(朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I);流速:0.8 m L·min-1;柱温:30℃。结果 7种成分的质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.999);平均加样回收率及相应的RSD分别为98.7%(1.6%)、97.9%(1.3%)、99.6%(0.8%)、97.7%(0.5%)、99.3%(1.4%)、96.9%(0.8%)和100.3%(0.6%)。结论本文作者建立的HPLC波长切换法同时测定龟鹿补肾胶囊中麦角甾苷、吉奥诺苷B1、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷I,为龟鹿补肾胶囊质量的全面评价提供了科学依据。Objective To develop simultaneous determination of seven components in Guilu Bushen capsule by HPLC gradient elution combined with wavelength switching method. Methods The Alltima C18 chromatographic column was used, and the mobile phase was acetonitrile -0. 2% phosphoric acid solution with gradient elution. Acteoside and jionoside B1 were detected at 330 nm, epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ were detected at 270 nm. The flow speed was 0. 8 mL·min^- 1, and the column temperature was 30 ℃. Results The good linearity between the concentration and the peak area was obtained for the 7 components in the determined ranges （ r 〉 0. 999 ）. The average recoveries and the corresponding RSD were 98.7% （ 1.6% ）, 97.9% （ 1.3% ）, 99.6% （0. 8% ）, 97.7% （0. 5% ）, 99.3% （ 1.4% ） ,96. 9% （0. 8% ） and 100. 3% （ 0. 6% ）, respectively. Conclusions A HPLC wavelength switching method has been successfully established for the simultaneous determination of acteoside, jionoside B1, epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ in Guilu Bushen capsule. The method is simple accurate and repeatable, which provides a scientific basis for the comprehensive evaluation of the quality of Guilu Bushen capsule.

关 键 词：龟鹿补肾胶囊 麦角甾苷 吉奥诺苷B1 朝藿定A 朝藿定B 朝藿定C 淫羊藿苷 宝藿苷I 

分 类 号：R917[医药卫生—药物分析学]