RP-HPLC-UV法同时测定兔儿伞中3种黄酮苷类成分的含量  

Simultaneous determination of three flavonoid glycosides in Syneilesis aconitifolia(Bunge) Maxim.by RP-HPLC-UV

作　　者：夏岩石[1] 杨建峰 韩成花 白玉华 仇维华 何法 XIA Yanshi1, YANG Jianfeng2, HAN Chenghua2, BAI Yuhua2, QIU Weihua2, HE Fa2(1. Department of Physical Education,Harbin Medical University,Daqing 163319, China;2. College of Pharmacy, Harbin Medical University, Daqing 163319, Chin)

机构地区：[1]哈尔滨医科大学大庆校区体育部,黑龙江大庆163319 [2]哈尔滨医科大学大庆校区药学院,黑龙江大庆163319

出　　处：《沈阳药科大学学报》2018年第3期221-225,共5页Journal of Shenyang Pharmaceutical University

基　　金：大庆市指导性科技计划项目(szdy-2015-07);哈尔滨医科大学创新科学研究资助项目(2017JCZX02)

摘　　要：目的建立反相高效液相色谱法同时测定兔儿伞药材中大豆苷、异槲皮苷和槲皮苷共3个黄酮苷类成分的含量。方法采用Hypersil ODS-2色谱柱(250 mm×4.6 mm,5μm),以乙腈-体积分数0.3%磷酸水溶液为流动相,进行梯度洗脱,流速为1.0 m L·min-1,检测波长360 nm,柱温30℃。结果标准曲线线性关系良好(r>0.999 2),精密度,稳定性和重复性实验均符合要求,加样回收率为97.38%~103.30%;对不同产地的兔儿伞药材进行了含量测定,上述3个黄酮苷类成分均能达到基线分离。结论本方法可用于兔儿伞药材的质量控制,同时对于兔儿伞药材的道地性分析也提供了科学依据。Objective To develop a method of high performance liquid chromatography for the content determination of daidzin, isoquercitrin and quercetin in Syneilesis aconitifolia （Bunge） Maxim.. Methods The analysis was performed on a Hypersil ODS-2 column （250 mm × 4. 6 mm, 5 μm） with gradient elution. The mobile phases were 0.3 % aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The column temperature was set at 30℃. Results The three marker flavonoid glycosides were separated well with good linearity （ r 〉 0.999 2）, precision, stability and repeatability. The recovery rate was 97.38% -103.30%. Three flavonoid glycosides in Syneilesis aconitifolia from different habitats were determined by HPLC-UV. Conclusions The method is simple, accurate and replicable and it can be used for the quality control as well as a scientific rationale for the Geo-authentication of Syneilesis aconitifolia （Bunge） Maxim..

关 键 词：兔儿伞 大豆苷 异槲皮苷 槲皮苷 黄酮苷 含量测定 

分 类 号：R917[医药卫生—药物分析学]