大鼠环杓后肌与腓肠肌去细胞支架的制备和比较研究  

Preparation and comparative study of decellularized rat cricoarytenoid dorsalis muscle and gastrocnemius muscle scaffold

作　　者：李术芹[1] 刘鹏飞 崔鹏程[1] LI Shuqin LIU Pengfei CUI Pengcheng(Department of Otolaryngology Head and Neck Surgery, Tangdu Hospital, Fourth Military Medical University, Xi＇an, 710038, Chin)

机构地区：[1]第四军医大学唐都医院耳鼻咽喉头颈外科,西安710038

出　　处：《临床耳鼻咽喉头颈外科杂志》2018年第5期360-364,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金(No:81271064)

摘　　要：目的:制备大鼠环杓后肌和腓肠肌的去细胞支架并比较二者的差异,为构建组织工程环杓后肌提供依据。方法:选取SD大鼠60只,随机分为6个组,包括5个实验组和1个对照组,每组10只。实验组分别为去细胞处理后2、4、6、8和10d组。经1%SDS溶液去细胞后,作大体观察、组织切片及苏木精-伊红、胶原纤维染色、扫描电镜观察并测量孔隙直径及面积,提取和检测DNA。对照组不作去细胞处理。结果:实验组大体上呈白色半透明状,对照组则色泽红润。实验4d组环杓后肌和8d组腓肠肌的肌纤维完全消失,细胞外基质结构保留良好,未检测出DNA。扫描电镜发现环杓后肌和腓肠肌的孔隙形态不同,且2组的孔隙直径差异有统计学意义(P<0.05),实验4d组环杓后肌支架的孔隙直径大于8d组腓肠肌支架。结论:与腓肠肌相比,环杓后肌去细胞支架具有孔隙形态及直径方面的优势,更适合作为组织工程环杓后肌的支架材料。Objective：To prepare and to compare the decellularized rat cricoarytenoid dorsalis muscle and gastrocnemius muscle scaffolds for providing a basis for tissue engineered cricoarytenoid dorsalis muscle.Method：Sprague Dawley rats were randomly divided into 6 groups with 10 rats in each group.The experimental groups were divided as 2,4,6,8,and 10 day groups after decellularization.Muscles were decellularized with 1% sodium dodecyl sulfate and observed by general observation,histological section with hematoxylin-eosin staining and collagenous fiber staining,scanning electron microscope images with measurement of pore diameter and area,and DNA extraction and quantification.The control group was not decellularized.Result：The decellularized muscles in experimental groups showed a semitransparent appearance while in control group the fresh muscle showed a ruddy color.Muscle fibers were completely disappeared in cricoarytenoid dorsalis muscle after 4 days decellularization and in gastrocnemius muscle after 8 days decellularzation,the extracellular matrix reserved well and no DNA was detected.Scanning electron microscope revealed a different pore size between decellularized cricoarytenoid dorsalis muscle and gastrocnemius muscle,and the difference was statistically significant.The pore diameter in 4 days decellularized cricoarytenoid dorsalis muscle was larger than the one in 8 days decellularzed gastrocnemius muscle.Conclusion：Compare to gastrocnemius muscle,decellularized cricoarytenoid dorsalis muscle has advantages in pore form and size and more suitable as a scaffold for tissue engineered cricoarytenoid dorsalis muscle.

关 键 词：组织工程 环杓后肌 去细胞支架 

分 类 号：R762[医药卫生—耳鼻咽喉科]