α-硫辛酸对6-羟多巴胺诱导PC12细胞损伤的保护作用  

Neuroprotective Effect of α-Lipoic Acid on PC12 Cells Induced by 6-hydroxydopamine

作　　者：李成朋 韩飞[1,2] 董智慧[1,2] 罗坤[4] 胡玉梅 官志忠[1,2,5] 吴昌学[1,2] 禹文峰[1,2] LI Chengpeng1,2 HAN Fei1,2, DONG Zhihui1,2 LUO Kun3 HU Yumei1,2 GUAN Zhizhong1,2,4, WU Changxue1,2, YU Wengfeng1,2(1.Key Laboratory of Molecular Biology of Guizhou Medical University, Guiyang 550004, Guizhou, China ; 2. Education Ministry Key Laboratory of Endemic and Minority Diseases of Guizhou Medical University, Guiyang 550004, Guizhou, China ; 3. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou, China ; 4. Department of Pathology of Guizhou Medical University, Guiyang 550004, Guizhou, Chin)

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵州医科大学地方病与少数民族疾病教育部重点实验室,贵州贵阳550004 [3]贵州医科大学 [4]贵阳中医学院基础部,贵州贵阳550002 [5]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2018年第3期264-268,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81360199);教育部科学技术研究项目(213032A);贵州省国际科技合作计划项目[黔科合外G字(2013)7026号];贵州省创新计划项目[黔教合协同创新中心(2014)06];贵州省教育厅项目[黔教合人才团队字(2015)63]

摘　　要：目的:研究α-硫辛酸(LA)对6-羟基多巴胺(6-OHDA)诱导PC12细胞损伤的保护作用。方法:体外培养PC12细胞,通过CCK-8实验筛选出诱导PC12细胞建立帕金森病(PD)细胞模型的6-OHDA最佳浓度及对PD细胞模型保护的LA最佳浓度,Western blot法检测LA预处理后对PD模型细胞中酪氨酸羟化酶(TH)表达的影响。结果:75μmol/L 6-OHDA作用于PC12细胞共培养18 h后,PC12细胞活力下降35%;而经10μmol/L LA预处理PC12细胞1 h后,再用75μmol/L 6-OHDA与PC12共培养18 h,则PC12细胞活力下降20%,提示75μmol/L 6-OHDA与10μmol/L LA为最佳处理浓度;经过LA的干预,6-OHDA诱导PC12建立的PD细胞模型中TH表达显著升高(P<0.05)。结论:LA对6-OHDA诱导的PC12细胞损伤具有保护作用。Objective： To evaluate the neuroprotective effect of α-lipoic acid on PC12 cells induced by 6-hydroxydopamine. Methods： PC12 cells were cultured in vitro. The optimal concentration of 6-OHDA and LA for the protection of PD cell modelbv was screened out by CCK-8 experiment,and the effect of TH expression in PC12 cells was detected by Western blot. Results： The viability of PC12 cells treated with 75 μmol/L 6-OHDA for 18 h decreased by 35%. After pretreatment with 10 μmol/L LA for 1 h,and co-culture of 75 μmol/L 6-OHDA with PC12 for 18 h,the viability of PC12 cells decreased by 20%. After LA intervention,the expression of TH in PD cells induced by 6-OHDA is significantly increased by PC12（ P 〈0. 05）. Conclusions： La has significant neuroprotective effects on 6-OHDA induced PC12 cell injury.

关 键 词：Α-硫辛酸 6-羟多巴胺 PC12细胞 酪氨酸羟化酶 帕金森病 神经保护 氧化应激 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]