甘肃一年生、二年生红芪和黄芪HPLC-ELSD指纹图谱比较  

HPLC-ELSD Fingerprint Comparison of One Year Old,Two Years Old Hedysari Radix and Astragalus Radix from Gansu

作　　者：许京[1] 叶迎[1] 王瑞海[1] 苗青[1] 刘丽梅[1] XU Jing, YE Ying, WANG Rui-hai, MIAO Qing, LIU Li-mei(Institute of Basic Theory Research of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, Chin)

机构地区：[1]中国中医科学院中医基础理论研究所

出　　处：《中国实验方剂学杂志》2018年第6期71-76,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院基本科研业务费自主选题项目(YZ-1438)

摘　　要：目的：建立甘肃一年生、二年生红芪和黄芪指纹图谱,对比研究红芪和黄芪指纹图谱相似度。方法：采用HPLCELSD,选用Agilent ZROBAX-SBC18色谱柱（4.6 mm×250 mm,5μm）,以水（A）-乙腈（B）为流动相,梯度洗脱,柱温35℃,进样体积15μL,Alltech ELSD 2000蒸发光检测器,漂移管温度110℃,载气流速3.0 L·min-1,分别建立一年生和二年生红芪各24批以及一年生和二年生黄芪各24批药材指纹图谱。结果：一年生、二年生红芪药材共有峰分别为8,7个,相似度分别为0.923,0.825,相同色谱峰6个,分别为毛蕊异黄酮苷,芒柄花苷,大豆皂苷Ⅰ,芒柄花素,异黄芪皂苷Ⅱ,黄芪皂苷Ⅰ;一年生、二年生黄芪药材共有峰分别为25,17个,相似度分别为0.980,0.997,相同色谱峰7个,分别为毛蕊异黄酮苷,芒柄花苷,毛蕊异黄酮,黄芪甲苷,黄芪皂苷Ⅲ,异黄芪皂苷Ⅱ,黄芪皂苷Ⅰ;一年生红芪与黄芪相似度为0.032,二年生红芪与黄芪相似度为0.023,共有色谱峰均为4个,分别为毛蕊异黄酮苷,芒柄花苷,异黄芪皂苷Ⅱ,黄芪皂苷Ⅰ。结论：在所确定的色谱条件下,一年生和二年生红芪和黄芪药材指纹图谱差异明显,可有效区分两种药材,二者共有成分少,相似度低,故红芪和黄芪不宜替代使用。Objective： To establish the fingerprint of one year old,two years old Hedysari Radix and Astragalus Radix from Gansu province,in order to compare the similarity between them. Method： By HPLCELSD,Agilent ZROBAX-SBC18（ 4. 6 mm × 250 mm,5 μm） chromatographic column was adopted,with A water and B acetonitrile as the mobile phase,and eluted in a gradient mode. The column temperature was set at 35 ℃,and the sample volume was 15 μL. Evaporative light detector was Alltech ELSD 2000,the drift tube temperature was 110 ℃,carrier gas flow rate was 3. 0 L·min-1. The fingerprint was established with 24 batches of Hedysari Radix and Astragalus Radix respectively. Result： The common peaks of one year old and two years old Hedysari Radix were 8 and 7,respectively,and the similarities were 0. 923 and 0. 825,respectively. There were six same peaks,which were respectively calycosin-7-glucoside,ononin,soybean saponin I,formononetin,isoastragalosideⅡ and astragaloside I. The common peaks of one year old and two years old Astragalus Radix were 25 and 17,respectively,and the similarities were 0. 980 and 0. 997,respectively. There were seven same peaks,which were calycosin-7-glucoside,ononin,calycosin,astragaloside Ⅳ,astragaloside Ⅲ,isoastragaloside Ⅱ and astragaloside I. The similarity was 0. 032 between one year old Hedysari Radix and Astragalus Radix,and was 0. 023 between two years old Hedysari Radix and Astragalus Radix. There were four common peaks,namely calycosin-7-glucoside,ononin and isoastragaloside Ⅱ and astragaloside I. Conclusion： The differences between one year old,two years old Hedysari Radix and Astragalus Radix medicinal herbs were obvious,and the two drugs could be effectively differentiated. There are a few common components between them, with a low similarity and great quality differences. Therefore,it is not suitable to substitute Hedysari Radix and Astragalus Radix with each other.

关 键 词：红芪 黄芪 指纹图谱 高效液相色谱-蒸发光散射法 

分 类 号：R284.1[医药卫生—中药学] R931.4[医药卫生—中医学]