Medicinal properties of Ocotea bullata stem bark extracts:phytochemical constituents,antioxidant and anti-inflammatory activity,cytotoxicity and inhibition of carbohydrate-metabolizing enzymes  

Medicinal properties of Ocotea bullata stem bark extracts:phytochemical constituents,antioxidant and anti-inflammatory activity,cytotoxicity and inhibition of carbohydrate-metabolizing enzymes

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作  者:Akintayo Lanre Ogundajo Lateef Apollo Adeniran Anofi Omotayo Ashafa 

机构地区:[1]Phytomedicine and Phytopharmacology Research Group,Department of Plant Sciences,University of the Free State,Qwaqwa Campus,Phuthaditjhaba 9866,South Africa

出  处:《Journal of Integrative Medicine》2018年第2期132-140,共9页结合医学学报(英文版)

摘  要:Objective: This study evaluates the phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity, and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark. Methods: Hexane, ethyl acetate, methanol and water were used to extract the air-dried sample. The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures. The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase, α-glucosidase and 5-1ipoxygenase enzymes respectively. Vero cells were employed to determine the cytotoxicity of the extracts. Results: The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while metha- nol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g rutin) contents than other extracts. Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19mg/mL), hydroxyl (25.77mg/mL) and 2,2-azino-bisC3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals, while ethyl acetate extract exhibited stronger inhibition (P 〈 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards. Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P 〈 0.05) compared to other extracts and gallic acid. The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose. There were no significant differences (P 〈 0.05) in half-maximum inhibitory concentration (ICso) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity. The aqueous extract was nontoxic to Vero cells with an IC50 value of 0.38 mg/mL. Conclusion: O. bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammatObjective: This study evaluates the phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity, and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark. Methods: Hexane, ethyl acetate, methanol and water were used to extract the air-dried sample. The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures. The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase, α-glucosidase and 5-1ipoxygenase enzymes respectively. Vero cells were employed to determine the cytotoxicity of the extracts. Results: The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while metha- nol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g rutin) contents than other extracts. Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19mg/mL), hydroxyl (25.77mg/mL) and 2,2-azino-bisC3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals, while ethyl acetate extract exhibited stronger inhibition (P 〈 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards. Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P 〈 0.05) compared to other extracts and gallic acid. The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose. There were no significant differences (P 〈 0.05) in half-maximum inhibitory concentration (ICso) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity. The aqueous extract was nontoxic to Vero cells with an IC50 value of 0.38 mg/mL. Conclusion: O. bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammat

关 键 词:Ocotea bullata Antioxidants Antidiabetics Anti-inflammatories Cytotoxicity 

分 类 号:R284[医药卫生—中药学] R285[医药卫生—中医学]

 

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