降钙素原荧光免疫层析方法的建立  

Detection of procalcitonin based on fluorescence immune chromatography

作　　者：刘传志[1] 李伟[1] 刘桂莹 杨羽[1] 宫平[1] 侯玥[1] Chuanzhi Liu, Wei Li, Guiying Liu, Yu Yang, Ping Gong, and Yue Hou(College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130021, Jilin, Chin)

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130021

出　　处：《生物工程学报》2018年第3期440-448,共9页Chinese Journal of Biotechnology

基　　金：吉林省教育厅"十三五"科学技术研究项目(吉教科合[2016]第385号)资助~~

摘　　要：降钙素原(Procalcitonin,PCT)是降钙素的前体物质,与人体细菌感染情况严重程度相关。通过PCT抗体与量子点偶联,自制试纸条,开发一种高灵敏度、快速便捷的PCT检测产品,对其初步应用。PCT单抗效价达107,PCT蛋白与量子点偶联,具有较好稳定性。试纸条线性检测范围0.15–120μg/L,灵敏度0.007μg/L,回收率范围91%–113%,批内批间变异系数小于8%。自制的荧光检测试纸条与市场上已有的降钙素原ELISA试剂盒进行比对,检测结果良好,基本能够完成临床样品的检测。Procalcitonin（PCT） is the precursor of calcitonin related to the severity of human bacterial infection. We made a test strip by coupling anti-PCT to quantum dot, in order to develop a highly sensitive and convenient PCT testing product. The anti-PCT titer had reached 10~7 because of the stability by coupling anti-PCT with quantum dot. The detecting linear range of the experiment was 0.15 to 120 μg/L, the sensitivity was 0.007 μg/L, the recovery range was 91% to 113%, and the intra-and inter-assay coefficient of variation was less than 8%. Comparing the homemade fluorescence-detected test strip with PCT ELISA kit on sale, we got accurate results which could mostly accomplish the test of clinical samples.

关 键 词：降钙素原 荧光免疫层析 量子点 试纸条 

分 类 号：R392-33[医药卫生—免疫学]